%0 Journal Article
%T Deltamethrin Inhibits the Human T-type Voltage-Sensitive Calcium Channel (Cav3.2)
%A Natasha Catlin
%A Steven B. Symington
%J Impulse : an Undergraduate Journal for Neuroscience
%D 2009
%I Appalachian State University Honors College
%X The goal of this study was to determine the effect of deltamethrin, a pyrethroid insecticide, on CaV3.2, a human T-type voltage-sensitive calcium channel expressed in Xenopus laevis (X.laevis) oocytes. Cav3.2 cDNA was transcribed into cRNA; the cRNA was then injected into X.laevis oocytes and electrophysiologically characterized using the two-electrode voltage clamp technique with Ba2+ as a charge carrier. Deltamethrin (10-7 M) reduced peak current in a nonreversible manner compared to the untreated control, but had no effect on the voltagedependent activation and inactivation kinetics. These findings confirm that human CaV3.2 is a target for deltamethrin and quite possibly other pyrethroid insecticides. These studies provide insight into the molecular mechanisms of the effect that pyrethroids have on voltage-sensitive calcium channels in general. This information will allow a more complete understanding of the molecular and cellular nature of pyrethroid-induced toxicity and expand our knowledge of the structure-activity relationships of pyrethroids with regard to their action on voltage-sensitive calcium channels.
%K Pyrethroids
%K Voltage-Sensitive
%K Calcium Channel
%K Xenopus laevis
%K Oocytes
%K Cav3.2
%U http://impulse.appstate.edu/sites/impulse.appstate.edu/files/2009_03_09_Catlin.pdf