%0 Journal Article
%T In vitro antiherpetic activity of a chemically sulfated galactomannan from Leucaena leucocephala seeds
%A LUCY ONO
%J Revista de Ci¨ºncias Farmac¨ºuticas B¨¢sica e Aplicada
%D 2010
%I Universidade Estadual Paulista
%X A chemically sulfated galactomannan (LLS-2) was obtained, starting from the polysaccharide extracted from seeds of Leucaena leucocephala, which presented 15.2% sulfate and a degree of sulfation of 0.60, and its effect on the replication of Herpes simplex virus 1 (HSV-1) in Vero cells was investigated. LLS-2 showed a 93.7% inhibition of the HSV-1 replication at a concentration of 2.5 micrograms/ml, when added during its adsorption step, evaluated by the plaque reduction method, and a selectivity index higher than 1 000, suggesting that it inhibits the HSV-1 binding to the host cell, but also, in a less significant way, it showed an antiviral effect following the virus attachment step, which indicates that sulfated polysaccharides could act by more than one antiviral mechanism. Keywords: Leucaena leucocephala. Galactomannan. Sulfation. HSV-1; Antiviral activity RESUMO Obteve-se uma galactomanana quimicamente sulfatada (LLS-2) a partir de polissacar¨ªdeo extra¨ªdo de sementes de Leucaena leucocephala, a qual apresentou 15.2% de sulfato e grau de derivatiza  o de 0,60, e, seu efeito antiviral sobre a replica  o do v¨ªrus Herpes simplex tipo 1 (HSV-1) em c¨¦lulas Vero foi avaliado pela metodologia de redu  o do n¨²mero de unidades formadoras de placas. LLS-2 apresentou 93.7% de inibi  o da replica  o viral ¨¤ concentra  o de 2,5 ¦Ìg/ml, quando adicionado durante as etapas iniciais de replica  o, com um ¨ªndice de seletividade maior que 1.000, sugerindo que LLS-2 inibe a liga  o de HSV-1 ¨¤s c¨¦lulas hospedeiras. Palavras-chave: Leucaena leucocephala. Galactomanana. Sulfata  o. HSV-1. Atividade antiviral.
%K Leucaena leucocephala
%K Galactomannan
%K Sulfation
%K HSV-1
%K Antiviral activity
%U http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/889