%0 Journal Article
%T Hemolytic activity and production of germ tubes related to pathogenic potential of clinical isolates of Candida albicans
%A MELYSSA FERNANDA NEGRI
%A MARIA GRACIELA FARIA
%A ELIANA GUILHERMETTI
%A ADRIANA ALTRˋO ALVES
%J Revista de Ci那ncias Farmac那uticas B芍sica e Aplicada
%D 2010
%I Universidade Estadual Paulista
%X ABSTRACT We assessed the virulence factor profile and in vitro antifungal susceptibility of 27 hospital isolates of C. albicans; 19 of these were from infections (16 urinary and three blood), and the other eight were isolated from sites of colonization (two from hands of health professionals, and six from central venous catheters). The virulence factors assayed were germ tube formation and production of extracellular products (hemolysins, proteinases, and phospholipases). Susceptibility to fluconazole, itraconazole, voriconazole and amphotericin B was determined by E-test. Regarding the virulence factors, the infection isolates produced significantly more hemolysin and germ tubes than the colonization isolates (p<0.05). There were no significant differences in the production of other factors between isolates from the two sources (p>0.05). Amphotericin B showed the lowest minimum inhibitory concentrations for all the isolates. The highest resistance was observed for the azoles, especially in the clinical isolates. These results suggest that the capacity of C. albicans to produce hemolysins and germ tubes may be associated with its pathogenic potential. Colonization isolates may pose a high risk of nosocomial infection, especially when the yeasts show resistance to antifungals. Keywords: Nosocomial infection. Virulence. Candida albicans. Germ tube. Hemolysins. RESUMO Atividade hemol赤tica e produ  o de tubos germinativos relacionados ao potencial patog那nico de isolados cl赤nicos de Candida albicans O perfil de virul那ncia e o de susceptibilidade in vitro aos antif迆ngicos de 27 amostras de C. albicans de origem hospitalar foi avaliado, sendo que 19 delas foram isoladas de infec  es (16 urin芍rias e tr那s sangu赤neas) e as outras oito foram isoladas de coloniza  o (duas de m os de profissionais da sa迆de e seis de cateter venoso central). Os seguintes fatores de virul那ncia foram investigados: forma  o de tubo germinativo e produ  o de compostos extracelulares (hemolisinas, proteinases e fosfolipases). Suscetibilidade ao fluconazol, itraconazol, voriconazol e anfotericina B foram determinadas por E-test. Em rela  o aos fatores de virul那ncia, os isolados de infec  o produziram significativamente mais hemolisina e tubos germinativos do que os de coloniza  o (p<0.05). N o houve diferen a significativa na produ  o das outras enzimas, entre os isolados das duas fontes (p>0.05). Anfotericina B mostrou as menores concentra  es inibit車rias m赤nimas para todos os isolados. Maiores 赤ndices de resist那ncia foram observados aos az車licos, especialmente entre os isolados cl赤nicos.
%K Nosocomial infection
%K Virulence
%K Candida albicans
%K Biofilms
%K Hemolysins
%U http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/886