%0 Journal Article
%T OPTIMALISATION OF SPECIES IDENTIFICATION OF COMMON CARP (CYPRINUS CARPIO) USING SYBR  GREEN I REAL-TIME PCR METHOD
%A Pavol Bajz¨ªk
%A Radoslav £¿idek
%A Jozef Golian
%A £¿ubom¨ªr Belej
%J Potravinarstvo : Scientific Journal for Food Industry
%D 2011
%I Association HACCP Consulting
%R 10.5219/154
%X European Union Member States, together with a number of countries around the world, places great emphasis on ensuring the protection of consumers as a potential food allergic from food allergies. to inform consumers that their product may contain any of the risk of allergenic ingredients. For species identification of fish and fish products as a potential food allergens are used by many analytical methods as well as their authentication. We are in our work applied the method of SYBR  Green I. Real-Time PCR. We focused on pre-designed molecular - genetic marker of common carp (Cyprinus carpio), which comes from the mtDNA control D - loop area. We analyzed its presence in DNA isolates from the 5 kinds of freshwater fish, diluted to 10 % concentration. Results of using the optimized SYBR   Green I Real-Time
%K species identification
%K SYBR  Green I. Real-Time PCR
%K Cyprinus carpio
%U http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/154