%0 Journal Article
%T Noncytopathic hepatitis A virus induces surface alterations in LLC-MK2 cells revealed by thin sections, negative staining, and scanning electron microscopy
%A Francisco Hern¨¢ndez-Chavarr¨ªa
%A Libertad Alzamora-Gonz¨¢lez
%A Libia Herrero-Uribe
%J Revista de Biolog¨ªa Tropical
%D 2002
%I Universidad de Costa Rica
%X Previous electron microscope studies of ultrastructural events during hepatitis A virus replication in experimentally infected cells have used only ultrathin section techniques. Nevertheless, no important differences were observed between infected and uninfected cells. This study was carried out using scanning electron microscopy and negative staining of whole LLC-MK2 cells grown directly on grids covered with support membranes, and then infected with an hepatitis A virus strain. Thin sections of infected and unifected controls were also analyzed. An intricate web of projections forming a net between cell interfaces was observed only in infected cells. Some of these projections were more than 700 nm long and had ballooning tips. Nevertheless, HAV particles were not visualized in the infected cells Los estudios previos sobre los eventos ultraestructurales en la replicaci¨®n del virus de la hepatitis Aen cultivos de c¨¦lulas infectadas experimentalmente, se han realizado exclusivamente mediante cortes ultrafinos. No obstante, no se han observado diferencias importantes entre c¨¦lulas infectadas y no infectadas. Este estudio fue realizado empleando microscopia electr¨®nica de rastreo, tinci¨®n negativa de c¨¦lulas (LLC-MK2) cultivadas directamente sobre rejillas para microscopia electr¨®nica de transmisi¨®n e infectadas con el virus de la hepatitis A. Adicionalmente se analizaron cortes ultrafinos de c¨¦lulas infectadas y no infectadas. Se observ¨® una intrincada red de proyecciones entre las superficies de las c¨¦lulas, que apareci¨® solo en las c¨¦lulas infectadas. Algunas de esas proyecciones med¨ªan m¨¢s de 700 nm de largo y terminaban en extremos abultados. Sin embargo, no se observaron viriones en las c¨¦lulas infectadas
%K Hepatitis A virus
%K negative staining
%K scanning electron microscopy
%K thin sections
%K ultrastructure
%U http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442002000200011