%0 Journal Article
%T Allelic Frequency of Kappa-Casein Locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole Grade (Bos indicus)
%A M. A. Mu¡¯in
%A A. Supriyantono
%J Media Peternakan
%D 2012
%I Bogor Agricultural University
%X This study was conducted to detect the genetic variants (single nucleotide polymorphism) of kappa-casein locus (Asp148/Ala) in F1: Simmental (Bos taurus) x Ongole grade (Bos indicus), SIMPO. Genomic DNA was isolated from blood sample of 40 SIMPO (21 males and 19 females). A 780 bp specific fragment of kappa-casein gene spanning from the forth exon region (517 bp) to forth intron (263 bp) was successfully amplified. The result of the PCR-RFLP (Polymerase Chain Reaction ¨C Restriction Fragment Length Polymorphisms) analysis using HindIII enzyme showed that two genotypes (AA and AB) were found at this locus in SIMPO. The frequencies of A and B alleles in SIMPO were 0.79 and 0.21, respectively. The frequency lies between B. taurus (Simmental) and B. indicus group.
%K gene
%K locus
%K kappa-casein
%K PCR-RFLP
%K SIMPO
%U http://medpet.journal.ipb.ac.id/index.php/mediapeternakan/article/view/6402