%0 Journal Article
%T Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon
%A Jiann-Horng Leu
%A Chih-Chin Chang
%A Jin-Lu Wu
%A Chun-Wei Hsu
%A Ikuo Hirono
%A Takashi Aoki
%A Hsueh-Fen Juan
%A Chu-Fang Lo
%A Guang-Hsiung Kou
%A Hsuan-Cheng Huang
%J BMC Genomics
%D 2007
%I BioMed Central
%R 10.1186/1471-2164-8-120
%X Sequencing of the complementary DNA clones of two libraries constructed from normal and WSSV-infected postlarvae produced a total of 15,981 high-quality ESTs. Of these ESTs, 46% were successfully matched against annotated genes in National Center of Biotechnology Information (NCBI) non-redundant (nr) database and 44% were functionally classified using the Gene Ontology (GO) scheme. Comparative EST analyses suggested that, in postlarval shrimp, WSSV infection strongly modulates the gene expression patterns in several organs or tissues, including the hepatopancreas, muscle, eyestalk and cuticle. Our data suggest that several basic cellular metabolic processes are likely to be affected, including oxidative phosphorylation, protein synthesis, the glycolytic pathway, and calcium ion balance. A group of immune-related chitin-binding protein genes is also likely to be strongly up regulated after WSSV infection. A database containing all the sequence data and analysis results is accessible at http://xbio.lifescience.ntu.edu.tw/pm/ webcite.This study suggests that WSSV infection modulates expression of various kinds of genes. The predicted gene expression pattern changes not only reflect the possible responses of shrimp to the virus infection but also suggest how WSSV subverts cellular functions for virus multiplication. In addition, the ESTs reported in this study provide a rich source for identification of novel genes in shrimp.White spot syndrome (WSS) is a highly contagious viral disease of penaeid shrimp. The cumulative mortality of diseased shrimp can reach 100% within 3¨C10 days. Since its first outbreak in 1993, WSS has caused serious economic losses to the shrimp farming industry worldwide. The causative agent, white spot syndrome virus (WSSV), is an enveloped, non-occluded, rod-shaped virus that contains a circular, double-stranded DNA of about 300 kb. This virus has an extremely wide range of potential hosts, infecting not only shrimps, but also other decapods [1,2
%U http://www.biomedcentral.com/1471-2164/8/120