%0 Journal Article
%T End-sequencing and characterization of silkworm (Bombyx mori) bacterial artificial chromosome libraries
%A Yoshitaka Suetsugu
%A Hiroshi Minami
%A Michihiko Shimomura
%A Shun-ichi Sasanuma
%A Junko Narukawa
%A Kazuei Mita
%A Kimiko Yamamoto
%J BMC Genomics
%D 2007
%I BioMed Central
%R 10.1186/1471-2164-8-314
%X More than 94000 BAC end sequences (BESs), comprising more than 55 Mbp and covering about 10.4% of the silkworm genome, were sequenced. Repeat-sequence analysis with known repeat sequences indicated that the long interspersed nuclear elements (LINEs) were abundant in BamHI BESs, whereas DNA-type elements were abundant in EcoRI BESs. Repeat-sequence analysis revealed that the abundance of LINEs might be due to a GC bias of the restriction sites and that the GC content of silkworm LINEs was higher than that of mammalian LINEs. In a BLAST-based sequence analysis of the BESs against two available whole-genome shotgun sequence data sets, more than 70% of the BESs had a BLAST hit with an identity of ¡Ý 99%. About 14% of EcoRI BESs and about 8% of BamHI BESs were paired-end clones with unique sequences at both ends. Cluster analysis of the BESs clarified the proportion of BESs containing protein-coding regions.As a result of this characterization, the identified BESs will be a valuable resource for genomic research on Bombyx mori, for example, as a base for construction of a BAC-based physical map. The use of multiple complementary BAC libraries constructed with different restriction enzymes also makes the BESs a more valuable genomic resource. The GenBank accession numbers of the obtained end sequences are DE283657¨CDE378560.The silkworm (Bombyx mori) has been domesticated for more than 5000 years because of the industrial importance of sericulture. Besides being used for silk production, the silkworm is also an effective host for the production of recombinant proteins and biomaterials [1-3]. It is also an important model organism of the Lepidoptera, the insect order that includes the majority of serious agricultural pests. Therefore, the accumulation of silkworm genome resources will be helpful for both the control of agricultural pests and the development of the silkworm as an industrial-scale resource of biomaterials or bioreactors.In silkworm, two individual whole-genome
%U http://www.biomedcentral.com/1471-2164/8/314