%0 Journal Article
%T Correlation of in silico and in vitro analysis of LuxS receptor with the crude extracts of Terminalia chebula retzius
%A Aishwarya. V
%A Kanimozhi. M
%A Sridhar. S
%J Advanced Biotech
%D 2013
%I 
%X The main objective of this paper is to compare and correlate in silico with the in vitro data obtained. Six organic extracts of Terminalia chebula retzius were extracted by simple extraction methodology using six solvents such as acetone, ethanol, methanol, petroleum ether, chloroform and ethyl acetate. Docking analysis using Autodock module present within PyRx 0.8 was performed to study the interaction between the target quorum sensing protein (LuxS) and two lead metabolites found in organic extracts of Terminalia chebula retzius. Gallic acid and Ellagic acid exhibited efficient molecular interaction with the target protein. Crude extracts were used to screen antibacterial effect by disc diffusion method against Bacillus subtilis. Methanolic and Ethanolic extracts were shown to exhibit high antibacterial activities (20mm each), followed by Acetone (18mm) and Ethyl acetate extract (16mm), while Chloroform and Petroleum ether exhibited no detectable zone of inhibition. The theoretical in silico studies results were found to support experimental anti-bacterial results.
%K Terminalia chebula retzius
%K Organic extracts
%K Docking
%K Disc Diffusion Assay
%U http://www.advancedbiotech.in/617.pdf