%0 Journal Article
%T Frecuencia espont芍nea en inducida de micron迆cleos transplacentarios en ratas Sprague Dawley Spontaneous and induced frequency of transplacental micronucleus in Sprague Dawley rats
%A D.F. Arencibia
%A L.A Rosario
%A Y.E Su芍rez
%A L Delgado
%J InVet
%D 2011
%I Universidad de Buenos Aires
%X El presente trabajo tuvo como objetivo determinar la frecuencia espont芍nea e inducida de micron迆cleos transplacentarios en ratas Sprague Dawley, demostrando el efecto clastog谷nico o aneug谷nico en el feto a trav谷s de la exposici車n materna y su vinculaci車n con el efecto genot車xico y reproductivo. Se formaron 4 grupos experimentales, 1) control negativo (simulacro), 2) control solvente NaCl (0,9%), 3) ciclofosfamida 50 mg/kg, y 4) bleomicina 30 mg/kg. Todos los grupos se administraron por v赤a intraperitoneal a los d赤as 14, 15 y 16 de la gestaci車n. A las 48 h posteriores a la 迆ltima inoculaci車n se procedi車 a realizar la eutanasia obteni谷ndose muestras de m谷dula 車sea materna y de h赤gado fetal. Se observ車 mayor inducci車n de da o en c谷lulas hep芍ticas fetales que en m谷dula 車sea materna. Se demostr車 que la ciclofosfamida es capaz de inducir mayor citotoxicidad y genotoxicidad que la bleomicina en ambos tipos celulares, demostrando el poder clastog谷nico transplacentario de ambos mut芍genos vinculando este ensayo de genotoxicidad a la reproducci車n. Adem芍s estos resultados se podr芍n utilizar en la evaluaci車n de nuevas drogas con car芍cter antigenot車xico por v赤a transplacentaria. The aim of this work were to determine the spontaneous and induced frequency of transplacental micronucleis in Sprague Dawley rats, demonstrating the clastogenic or aneugenic effects in the fetus through the maternal exposure, and their linking with the genotoxic and reproductive effect. Four experimental groups were formed, 1) negative control (mockery), 2) solvent control NaCl (0,9 %), 3) cyclophosphamide to 50 mg/kg, and 4) bleomycin to 30 mg/kg. All the groups were administered the 14, 15 and 16 days of the gestation by intraperitoneal route. 48 h to the last inoculation was preceded to the euthanasia, being obtained the maternal bone marrow and fetal liver samples. Bigger induction of damage was observed in fetal hepatic cells when it was compared with maternal bone marrow cells. It was demonstrated that the cyclophosphamide is able to induce bigger citotoxicity and genotoxicity that the bleomycin in both cellular types, demonstrating the transplacental clastogenic potential of both mutagens linking this genotoxicity assay to the reproduction. These results are used in the new drugs evaluation with antigenotoxic effects by transplacental route.
%K Micron迆cleos transplacentarios
%K Ratas Sprague Dawley
%K Genotoxicidad
%K Embarazo
%K Transplacental micronucleus
%K Sprague Dawley rats
%K Genotoxicity
%K Pregnancy
%U http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982011000100006