%0 Journal Article
%T Estructura y la ultraestructura del ovario de Cichlasoma urophthalmus (Perciformes: Cichlidae)
%A Rub赤 Viedma
%A Jonathan Franco
%A Carlos Bedia
%A Guadalupe Guedea Fern芍ndez
%J Revista de Biolog赤a Tropical
%D 2011
%I Universidad de Costa Rica
%X Se realiz車 un estudio descriptivo de las g車nadas femeninas de Cichlasoma urophthalmus. Las muestras fueron recolectadas en la Laguna de Alvarado Veracruz, M谷xico en el per赤odo 2007-2008 que incluy車 las temporadas de Nortes, Secas y Lluvias. Se extrajeron las g車nadas femeninas y una parte se fij車 en formol al 4% para su tratamiento por t谷cnica histol車gica de rutina hematoxilina y Eosina (H-E) y otra parte se proces車 para microscopia electr車nica de transmisi車n. Las g車nadas se fijaron en glutaraldeh赤do al 3% y OsO4 al 2%, se deshidrataron en etanol de 50 al 100% para ser incluidas en Ep車n. Se realizaron cortes finos y semifinos contrastados con citrato de plomo y acetato de uranilo. Los ovarios de C. urophthalmus son pareados presentan un desarrollo asincr車nico con ovocitos previtelog谷nicos en estadio perinuclear tard赤o, asociados a las lamelas ov赤geras y ovocitos vitelog谷nicos del VII estadio, 谷stos 迆ltimos presentan una zona radiada bien definida, con gr芍nulos de v赤telo lip赤dico y ves赤culas de v赤telo proteico que se distribuyen en capas conc谷ntricas, que rodean al n迆cleo. El presente estudio, permiti車 conocer m芍s a fondo los cambios de la estructura y ultraestructura de los componentes de los fol赤culos ov芍ricos, en C. urophthalmus durante las diferentes etapas de crecimiento de los oocitos. Structure and ultrastructure of the ovary of Cichlasoma urophthalmus (Osteichthyes: Cichlidae). The study of the normal development, differentiation, structure and function of various components of developing follicles in the ovaries of numerous fish species have been a consistent focus of comparative reproduction. The structural and ultrastructural features of gonads from Cichlasoma urophthalmus have received scarce attention. In this work, we realized a descriptive study of female gonads of Cichlasoma urophthalmus. A total of 40 samples were collected in the Veracruz Alvarado Lagoon, Mexico in 2007-2008 period including the windy, dry and rainy seasons. Female gonads were extracted and a portion was fixed in 4% formaldehyde for treatment for routine histology hematoxylin and eosin (HE) and another part was processed for transmission electron microscopy (TEM). The gonads were fixed in 3% glutaraldehyde and 2% osmium tetroxide, followed by dehydrated in ethanol 50%, 70%, 80%, 95% and 100% for inclusion in Epon, thin sections were then prepared and were contrasted with lead citrate and uranyl acetate. The process of oocyte development can be divided into five distinct stages (formation of oocytes from oogonia, primary growth, lipid stage, vitellogenesis and maturation)
%K C. urophthalmus
%K ovarios
%K estructura y ultraestructura
%K C. urophthalmus
%K ovaries
%K estructure and ultraestructure
%K oocyte
%U http://www.scielo.sa.cr/scielo.php?script=sci_arttext&pid=S0034-77442011000200016