%0 Journal Article
%T Glycogenome expression dynamics during mouse C2C12 myoblast differentiation suggests a sequential reorganization of membrane glycoconjugates
%A Mathilde Janot
%A Aymeric Audfray
%A C¨Śline Loriol
%A Agn¨¨s Germot
%A Abderrahman Maftah
%A Fabrice Dupuy
%J BMC Genomics
%D 2009
%I BioMed Central
%R 10.1186/1471-2164-10-483
%X Of the 276 genes expressed, 95 exhibited altered mRNA expression when C2C12 cells differentiated and 37 displayed more than 4-fold up- or down-regulations. Principal Component Analysis and Hierarchical Component Analysis of the expression dynamics identified three groups of coordinately and sequentially regulated genes. The first group included 12 down-regulated genes, the second group four genes with an expression peak at 24 h of differentiation, and the last 21 up-regulated genes. These genes mainly encode cell adhesion molecules and key enzymes involved in the biosynthesis of glycosaminoglycans and glycolipids (neolactoseries, lactoseries and ganglioseries), providing a clearer indication of how the plasma membrane and extracellular matrix may be modified prior to cell fusion. In particular, an increase in the quantity of ganglioside GM3 at the cell surface of myoblasts is suggestive of its potential role during the initial steps of myogenic differentiation.For the first time, these results provide a broad description of the expression dynamics of glycogenes during C2C12 differentiation. Among the 37 highly deregulated glycogenes, 29 had never been associated with myogenesis. Their biological functions suggest new roles for glycans in skeletal myogenesis.Myogenesis is a complex process which leads muscle progenitor cells to proliferate and then differentiate into myotubes. This process is strongly controlled by the spatio-temporal expression of myogenic regulatory factors (MRFs) - MyoD, Myf5, myogenin and Mrf4 (or Myf6) [1,2] - and by several transcription factors of the myocyte enhancer factor-2 (MEF2) family [3]. Their expression defines different stages in the myogenic process: myoblast proliferation, cell-cycle withdrawal, cell fusion to form myotubes, and the maturation of myotubes into myofibers. MRFs are members of the bHLH (basic Helix-Loop-Helix) protein family [4]. They cooperate with MEF2 transcription factors to mediate the transcription of muscle-spe
%U http://www.biomedcentral.com/1471-2164/10/483