%0 Journal Article
%T An expression database for roots of the model legume Medicago truncatula under salt stress
%A Daofeng Li
%A Zhen Su
%A Jiangli Dong
%A Tao Wang
%J BMC Genomics
%D 2009
%I BioMed Central
%R 10.1186/1471-2164-10-517
%X The results of our microarray experiment with roots of M. truncatula under 180 mM sodium chloride were deposited in the MtED database. Additionally, sequence and annotation information regarding microarray probe sets were included. MtED provides functional category analysis based on Gene and GeneBins Ontology, and other Web-based tools for querying and retrieving query results, browsing pathways and transcription factor families, showing metabolic maps, and comparing and visualizing expression profiles. Utilities like mapping probe sets to genome of M. truncatula and In-Silico PCR were implemented by BLAT software suite, which were also available through MtED database.MtED was built in the PHP script language and as a MySQL relational database system on a Linux server. It has an integrated Web interface, which facilitates ready examination and interpretation of the results of microarray experiments. It is intended to help in selecting gene markers to improve abiotic stress resistance in legumes. MtED is available at http://bioinformatics.cau.edu.cn/MtED/ webcite.Legumes are important economic crops that provide humans with food, livestock with feed, and industry with raw materials [1]. Additionally, legumes can fix nitrogen with rhizobia in soil, and the plants do not require external nitrogen sources such as nitrogen fertilizers [2]. Medicago truncatula (the barrel medic) has been selected as a model legume because it is self-fertile and diploid, and has a short life cycle and a relatively small genome [3-5]. The genome of the cultivar Jemalong line A17 is currently being sequenced by the Medicago Genome Sequencing Consortium (MGSC) [6].Plant growth and crop productivity is largely limited by environmental factors that include water-deficit stress, such as by salinity, which bring about large-scale alterations in gene expression in plants [7]. High NaCl concentrations in soil cause salinity stress and limit the geographical distribution of most highly salinity-sens
%U http://www.biomedcentral.com/1471-2164/10/517