%0 Journal Article
%T EVALUACI車N IN VITRO DEL POTENCIAL PROBI車TICO DE BACTERIAS 芍CIDO L芍CTICAS AISLADAS DE SUERO COSTE O IN VITRO EVALUATION OF PROBIOTIC POTENTIAL OF LACTIC BACTERIA ACID ISOLATED FROM COASTAL SERUM
%A Mar赤a C. Cueto-Vigil
%A Yudtanduly Acuˋa-Monsalve
%A Jacqueline Valenzuela-Riaˋo
%J Actualidades Biol車gicas
%D 2010
%I Universidad de Antioquia
%X Un grupo de 53 bacterias 芍cido l芍cticas (BAL) aisladas del suero coste o, fueron sometidas a estudios preliminares in vitro simulando las condiciones del tracto digestivo, para determinar sus caracter赤sticas como potenciales probi車ticos: se evalu車 la tolerancia a pH 芍cido (MRS pH: 2,0) y sales biliares (MRS con sales biliares al 0,3%) y posteriormente se determin車 la poblaci車n sobreviviente como log UFC/ml. A las cepas tolerantes a las condiciones mencionadas, se les determin車 la resistencia a 14 antibi車ticos de uso comercial, se evalu車 la adhesi車n a mucus intestinal y la producci車n de 芍cido l芍ctico por cromatograf赤a l赤quida de alta precisi車n (HPLC). Se encontr車 que 54,7% de las BAL evaluadas son tolerantes a las condiciones de pH 芍cido y 49,1 a 0,3% de sales biliares con una poblaci車n de 10(6) log UFC/ml en promedio. Siete cepas fueron seleccionadas por presentar sensibilidad a vancomicina antibi車tico de importancia epidemiol車gica y se adhirieron a mucus intestinal, reuniendo las condiciones requeridas para considerarse como potencialmente probi車ticas. Adicionalmente la cuantificaci車n de 芍cido l芍ctico para las cepas seleccionadas present車 un rango entre 0,13 ㊣ 0,05 y 1,0 ㊣ 0,08 g/l. A group of 53 lactic acid bacteria (LAB) isolated from costal serum were evaluated with preliminary in vitro studies simulating the conditions of the digestive tract, to determine their characteristics as potential probiotics; acid pH tolerance (MRS pH: 2.0) and bile salts (MRS with 0.3% bile salts) and subsequently the surviving population was determined as log CFU/ml. Strains tolerant to such conditions were evaluated for resistance to 14 antibiotics of commercial use; we assessed adherence to intestinal mucus and the production of lactic acid by high performance liquid chromatography (HPLC). It was shown that 54.7% of the LAB evaluated were tolerant to acid pH conditions and 49.1 to 0.3% bile salts with a population of 10(6) log CFU/ml on average. Seven strains were selected by antibiotic vancomycin tenderness of epidemiological importance and adhered to intestinal mucus, meeting the requirements to be considered as potentially probiotic. Additionally, the quantification of lactic acid in the selected strains showed a range between 0.13 and 1.0 ㊣ 0.05 ㊣ 0.08 g/l.
%K 芍cido l芍ctico
%K bacterias 芍cido l芍cticas
%K probi車tico
%K resistencia a pH
%K sales biliares
%K suero coste o
%K acid pH and bile salt resistance
%K coastal serum
%K lactic acid
%K lactic acid bacteria
%K probiotic
%U http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0304-35842010000200001