%0 Journal Article
%T Am¨¦lioration du taux de multiplication in vitro de Jatropha curcas L
%A Mezda Mve
%A SD.
%A Mergeai
%A G.
%A Baudoin
%A JP.
%A Toussaint
%A A.
%J Tropicultura
%D 2010
%I Agri-Overseas
%X Improvement of the Rate of in vitro Multiplication of Jatropha curcas L. In order to improve the in vitro multiplication rate (number of shoots/explant/subculture) of Jatropha curcas L. axillary nodes taken from young plants of two accessions of this species (originating from Cameroon and Senegal) have been cultivated for three weeks on a MS medium supplemented with 8.87 ¦ÌM BAP, 4.92 ¦ÌM IBA, and 30 g/l sucrose at pH 5.7 ¡À 0.1, and solidified with 0.7% agar. The shoots obtained from each original explant were then transferred to proliferation media (PM) consisting of MS medium supplemented with 2.21 to 8.9 ¦ÌM BA or 2.21 to 8.9 ¦ÌM kinetin in combination with 2.46 ¦ÌM IBA. Each combination was completed with 33.12 ¦ÌM adenine sulfate, 82.92 ¦ÌM of glutamine and 30 g / L sucrose. The best multiplication rate was obtained for the PM medium containing 6.65 ¦ÌM BA and 2.46 ¦ÌM AIB. On this medium 42.72 ¡À 3.22 and 38.15 ¡À 4.7 shoots/explant were obtained respectively for the accessions from Cameroon and Senegal after 6 weeks of culture, and the mean multiplication rates were 8.27 ¡À 1.27 (accession from Cameroon) and 7.89 ¡À 1.13 (accession from Senegal) shoots per explant during the 7 following subcultures (3 weeks/subculture). This medium was also the one that allowed the best overall growth in shoot height. Leafy shoots obtained have been rooted in a medium containing half of the major mineral components of MS supplemented with 5.7 ¦ÌM IBA, 1.5% sucrose and solidified with 0.7% agar, then acclimated with a survival rate of 97%. These results allow considering the establishment of industrial units of plantlet multiplication from elite clones of Jatropha curcas.
%K Jatropha curcas L.
%K Rate multiplication
%K accessions
%K in vitro culture
%K nodal explants
%K Cameroon
%K Senegal
%K Belgium
%U http://www.tropicultura.org/text/v28n4/200.pdf