%0 Journal Article
%T Effects of Bisphenol A on apoptosis of spermatogenic cells and expression of Bax and Bcl-2 proteins in the Siberian frog Rana chensinensis
%A LIN Sheng-Nan
%A ZHANG Yu-Hui
%J Acta Zoologica Sinica
%D 2008
%I 
%X In order to investigate the effects of bisphenol A (BPA) on apoptosis and the expression levels of Bax and Bcl-2 protein in amphibian spermatogenic cells, we treated Rana chensinensis with concentrations of 10-7, 10-6, 10-5mol/L BPA respectively. Testes were removed after R.chensinensis had been treated for 3 , 5 or 7 days. Apoptotic cells were examined by TUNEL and Methyl Green-Pyronine. The expression levels of Bax and Bcl-2 protein in spermatogenic cells were detected by immunohistochemical staining. The results show that apoptotic index £¨AI£©of spermatogenic cells of R.chensinensis treated with BPA is significantly higher than that of the control groups. AI of spermatogenic cells in 10-6 and 10-7 mol/L BPA groups are similar (P>0.05), but AI in 10-5 mol/L is significantly higher than that of in 10-6 and 10-7 mol/L BPA groups (P<0.05). AI of spermatogenic cells increases with prolonged treatment. The relative intensity of Bax immunostaining in BPA treated groups is higher than that of control groups (P<0.05) while the relative intensity of Bcl-2 in BPA treated groups is lower than that of control groups (P<0.05). Positive correlations exist between the AI of spermatogenic cells and the expression ratio of Bax/Bcl-2. These results indicate that BPA can enhance the apoptosis of spermatogenic cells in R.chensinensis, and that the change of the expression ratio of Bax /Bcl-2 might have caused the increased apoptosis of spermatogenic cells in BPA treated R. chensinensis [Acta Zoologica Sinica 54(5): 867¨C876, 2008].
%K Siberian frog
%K Rana chensinensis
%K Bisphenol A
%K Spermatogenic cell
%K Cell apoptosis
%K Bax protein
%K Bcl-2 protein
%U http://www.actazool.org/paperdetail.asp?id=10958