%0 Journal Article
%T Effects of Dexamethasone on the proliferation and apoptosis of swine kidney fibroblast induced by TRAIL
%A LI Xin
%A YANG Gong-She
%A JIA Qing
%A GUAN Wei-Jun
%J Acta Zoologica Sinica
%D 2008
%I 
%X 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetraz-olium bromide (MTT) assay was applied to measure the cell growth. And flow cytometry (FCM) was adopted to detect the changes of FRSs cell cycle and apoptosis rate .In addition, the Semiquantitative RT-PCR was using to assessed the regulation of dexamethasone(DEX) for Osteoprotegerin(OPG) or Ligand of receptor activator of nuclear factor kappa B(RANKL) in FRS. The results showed that TRAIL could prompt the growth of swine kidney fibroblast at the concentrations of 0.01每5 mg/L, but TRAIL inhibited the growth of FRS at the concentrations of 10每50 mg/L, but at the concentrations of 10每50 mg/L, TRAIL inhibited the growth of swine kidney fibroblast and even led them to apoptosis. DEX could regulate the effects of cytokines. The effect of DEX on TRAIL is synergies and the effective concentrations of DEX are 10-6每10-10 mol/L. Cell cycle analysis and apoptosis detection showed that the peak concentration of TRAIL inducing FRSs＊ proliferation was 5 mg/L. Under this concentration, TRAIL(5 mg/L)+DEX (10-8 mol/L) group showed that the proliferation index of FRSs (Prl.) was increased by 2.49% compared with TRAIL 5 mg/L group(P<0.05); TRAIL10 mg/L showed that the proliferation index apoptosis rate of FRSs was not significant. But TRAIL(10 mg/L) + DEX(10-8 mol/L) group showed that the G0/G1 phase was increased by 2.36%(P<0.05) and the apoptosis rate was increased by 6.79%(P<0.01) compared with TRAIL 10 mg/L group. Semi-quantitative RT-PCR showed that DEX could inhibit the constitutive OPG and stimulate RANKL mRNA steady-state levels in a dose-dependent manner. These results indicate that TRAIL prompt not only the growth but also the apoptosis of FRSs which depends on the concentrations of TRAIL. The phenomenon is related to the change of cell cycle of FRSs induced by TRAIL and DEX. The OPG/RANK/RANKL system is an important pathway that DEX can mediate the effects of TRAIL on proliferation and apoptosis of FRSs[Acta Zoologica Sinica 54(5): 836 每 845, 2008].
%K Tumor necrosis factor (TNF)-related apoptosis-inducing ligand(TRAIL)
%K Osteoprotegerin(OPG)
%K Dexamethasone
%K Fibroblast
%K Apoptosis
%U http://www.actazool.org/paperdetail.asp?id=10954