%0 Journal Article
%T Yeast glucose pathways converge on the transcriptional regulation of trehalose biosynthesis
%A Eva Apweiler
%A Katrin Sameith
%A Thanasis Margaritis
%A Nathalie Brabers
%A Loes van de Pasch
%A Linda V Bakker
%A Dik van Leenen
%A Frank CP Holstege
%A Patrick Kemmeren
%J BMC Genomics
%D 2012
%I BioMed Central
%R 10.1186/1471-2164-13-239
%X In general, the mutations do not induce pathway-specific transcriptional responses. Instead, one main transcriptional response is discerned, which varies in direction to mimic either a high or a low glucose response. Detailed analysis uncovers established and new relationships within and between individual pathways and their members. In contrast to signalling components, metabolic components of the glucose regulatory system are transcriptionally more frequently affected. A new network approach is applied that exposes the hierarchical organisation of the glucose regulatory system.The tight interconnection between the different pathways of the glucose regulatory system is reflected by the main transcriptional response observed. Tps2 and Tsl1, two enzymes involved in the biosynthesis of the storage carbohydrate trehalose, are predicted to be the most downstream transcriptional components. Epistasis analysis of tps2жд double mutants supports this prediction. Although based on transcriptional changes only, these results suggest that all changes in perceived glucose levels ultimately lead to a shift in trehalose biosynthesis.
%K Regulatory networks
%K Glucose signalling
%K Trehalose biosynthesis
%K Gene expression profiling
%K Saccharomyces cerevisiae
%U http://www.biomedcentral.com/1471-2164/13/239/abstract