%0 Journal Article
%T Simultaneous Multicomponent Analysis of Ampicillin and Probenecid in Pharmaceutical formulation by Reverse Phase High Performance Liquid Chromatography
%A Thomas Kurian*
%A Jose Kurien
%A Helen William and Bijumol C.
%J Hygeia : Journal for Drugs and Medicines
%D 2012
%I 
%X Plan: Reverse Phase High Performance Liquid Chromatographic method for Ampicillin and Probenecid describedPrologue: Ampicillin trihydrate is an antibiotic active against mainly gram positive bacteria and some gram negative bacteria. Probenecid is a uricosuric agent used in gout therapy. The present study aims at the development of Reverse phase High Performance Liquid Chromatography method for simultaneous estimation of Ampicillin and Probenecid in combined dosage formulations.Methodology: The HPLC system used consists of Waters 501 pump and a Waters 486 tunable wave length UV-visible detector. The data station was computer controlled using base line 810 software which includes an integrator and a recorder. The mobile phase used was sodium acetate solution (50mm): acetonitrile (75:25). The UV response was measured at 235nm. The column used was Reverse phase(5 micron) 25cm x4.6mm. Outcome: The method provided adequate accuracy and precision .The developed RP-HPLC method can be used for the routine analysis of Ampicillin and Probenecid in combination.
%K Ampicillin
%K Probenecid
%K Reverse phase
%K High performance liquid chromatography.
%U http://www.hygeiajournal.com/Downloads/155126018565-69%20Thomas%20hplc%20public%20final.pdf