%0 Journal Article
%T Localization of acid phosphatase activity in the apoplast of root nodules of pea (Pisum sativum)
%A Marzena Sujkowska
%A Wojciech Borucki
%A WŁżadysŁżaw Golinowski
%J Acta Societatis Botanicorum Poloniae
%D 2006
%I Polish Botanical Society
%R 10.5586/asbp.2006.006
%X Changes in the activity of acid phosphatase (AcPase) in the apoplast of pea root nodule were investigated. The activity was determined using lead and cerium methods. The results indicated a following sequence of AcPase activity appearance during the development of the infection thread: 1) low AcPase activity appears in the outer part of cells of symbiotic bacteria; 2) bacteria show increased AcPase activity, and the enzyme activity appears in the thread walls; 3) activity exhibits also matrix of the infection thread; 4) bacteria just before their release from the infection threads show high AcPase activity; 5) AcPase activity ceases after bacteria transformation into bacteroids. The increase in bacterial AcPase activity may reflect a higher demand for inorganic phosphorus necessary for propagation of the bacteria within the infection threads and/or involved in bacteria release from the infection threads.
%K acid phosphatase
%K localization
%K infection thread
%K nodule
%K Pisum sativum
%U http://pbsociety.org.pl/journals/index.php/asbp/article/view/516