%0 Journal Article
%T Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation
%A Magda Grabsztunowicz
%A Grzegorz Jackowski
%J Acta Societatis Botanicorum Poloniae
%D 2013
%I Polish Botanical Society
%R 10.5586/asbp.2012.043
%X A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on Percoll gradient step. The intactness and purity of the chloroplasts isolated from leaves of low irradiance-acclimated plants by using this protocol (confirmed by phase contrast microscopy as well as enzymatic and immunological approaches) allows plausible studies on low irradiance-dependent Rubisco regulation.
%K acclimation
%K chloroplast isolation
%K differential centrifugation
%K low irradiance
%K Arabidopsis thaliana
%K Rubisco
%U http://pbsociety.org.pl/journals/index.php/asbp/article/view/1233