%0 Journal Article
%T A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers
%A Alessandra C Palhares
%A Taislene B Rodrigues-Morais
%A Marie-Anne Van Sluys
%A Douglas S Domingues
%A Walter Maccheroni
%A Hamilton Jord£¿o
%A Anete P Souza
%A Thiago G Marconi
%A Marcelo Mollinari
%A Rodrigo Gazaffi
%A Antonio Augusto F Garcia
%A Maria Lucia Carneiro Vieira
%J BMC Genetics
%D 2012
%I BioMed Central
%R 10.1186/1471-2156-13-51
%X The mapping population parents (¡®IAC66-6¡¯ and ¡®TUC71-7¡¯) contributed equally to polymorphisms, independent of marker type, and generated markers that were distributed into nearly the same number of co-segregation groups (or CGs). Bi-parentally inherited alleles provided the integration of 19 CGs. The marker number per CG ranged from two to 39. The total map length was 4,843.19£¿cM, with a marker density of 8.87£¿cM. Markers were assembled into 92 CGs that ranged in length from 1.14 to 404.72£¿cM, with an estimated average length of 52.64£¿cM. The greatest distance between two adjacent markers was 48.25£¿cM. The scIvana_1-based markers (56) were positioned on 21 CGs, but were not regularly distributed. Interestingly, the distance between adjacent scIvana_1-based markers was less than 5£¿cM, and was observed on five CGs, suggesting a clustered organization.Results indicated the use of a NBS-profiling technique was efficient to develop retrotransposon-based markers in sugarcane. The simultaneous maximum-likelihood estimates of linkage and linkage phase based strategies confirmed the suitability of its approach to estimate linkage, and construct the linkage map. Interestingly, using our genetic data it was possible to calculate the number of retrotransposon scIvana_1 (~60) copies in the sugarcane genome, confirming previously reported molecular results. In addition, this research possibly will have indirect implications in crop economics e.g., productivity enhancement via QTL studies, as the mapping population parents differ in response to an important fungal disease.
%K Saccharum spp
%K AFLP
%K EST-SSR
%K Retrotransposon-based markers
%K Single-dose markers
%K Integrated genetic map
%K Marker distribution
%U http://www.biomedcentral.com/1471-2156/13/51/abstract