%0 Journal Article
%T Quantification of argyrophillic, argentaffin and insulin immunoreactive cells in the small intestine in the opossum Didelphis aurita (Wied-Neuwied, 1826) = Quantifica  o das c¨¦lulas argir¨®filas, argentafins e imunorreativas ¨¤ insulina nos diferentes segmentos do intestino delgado do gamb¨¢ Didelphis aurita (Wied-Neuwied, 1826)
%A Gl¨¢ucia Marques Freitas-Ribeiro
%A Cl¨¢udio C¨¦sar Fonseca
%A Sirlene Souza Rodrigues
%A S¨¦rgio Luis Pinto da Matta
%J Acta Scientiarum : Biological Sciences
%D 2011
%I Eduem - Editora da Universidade Estadual de Maring¨¢
%X The objective of this study was to quantify argyrophillic, argentaffin and insulin immunoreactive endocrine cells in the different segments of the small intestine of Didelphis aurita and measure probable differences in the number of these cells between adult and post-pubertal animals. Biological material consisted of ten male and female opossums specimen, divided in two groups according to weigh. The utilized staining techniques were Grimelius, modified Masson- Fontana and direct immunoperoxidase. Results indicated a predominance of argyrophillic cells in the small intestine of opossums from class 1 and 2, with an average of 52.58 and 56.15 cells mm-2, respectively; of which, the average number of total endocrine cells, argyrophillic and argentaffin cells decreased distally in the intestinal segments of opossums from classes 1 and 2. No significant difference was observed for the insulin immunoreactive cells between the intestinal segments of animals from class 2. A greater number of insulin immunoreactive cells was encountered in the jejunum and ileum of animals from class 2 when compared to the same segment in animals from class 1. Os objetivos deste trabalho foram quantificar as c¨¦lulas end¨®crinasargir¨®filas, argentafins e imunorreativas ¨¤ insulina nos diferentes segmentos do intestino delgado de gamb¨¢s Didelphis aurita e mensurar prov¨¢veis diferen as no n¨²mero destas c¨¦lulas entre animais adultos e p¨®s-p¨²beres. Dez exemplares de gamb¨¢s D. aurita machos e f¨ºmeas foramdivididos em dois grupos de acordo com o peso. As t¨¦cnicas de colora  o utilizadas foram Grimelius, Masson-Fontana modificado e Imunoperoxidase direta. Os resultados indicaram um predom¨ªnio das c¨¦lulas argir¨®filas no intestino delgado de gamb¨¢s da classe 1 e 2, com uma m¨¦dia de 52,58 e 56,15 c¨¦lulas mm-2, respectivamente. O n¨²mero m¨¦dio de c¨¦lulas end¨®crinas totais, c¨¦lulas argir¨®filas e argentafins decresceu distalmente nos segmentos intestinais dos gamb¨¢s das classes 1 e 2. Nenhuma diferen a significativa foi observada para as c¨¦lulas imunorreativas ¨¤ insulina entre os segmentos intestinais dos animais da classe 2. Foi encontrado maior n¨²mero de c¨¦lulas imunorreativas ¨¤ insulina no jejuno e ¨ªleo de animais da classe 2 quando comparado ao mesmo segmento em animais da classe 1.
%K digestive tract
%K endocrine cells
%K immunoreactive cells
%K mammal
%K marsupial
%K trato digestivo
%K c¨¦lulas end¨®crinas
%K c¨¦lulas imunorreativas
%K mam¨ªferos
%K marsupial
%U http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/8644/8644