%0 Journal Article
%T The Apoptotic Effects of Cisplatin on Human Colon Cancer Cell Line (HT29)
%A Venhar G¨¹RB¨¹Z
%A Ak£¿n YILMAZ
%A £¿zlem G£¿K£¿E
%A Ece KONA£¿T£¿bbi Biyoloji ve Genetik Anabilim Dal£¿
%A T£¿p Fak¨¹ltesi
%A Gazi ¨¹niversitesi
%A Ankara
%A Turkey
%J Marmara Medical Journal
%D 2011
%I Marmara University
%X Objective: In this study, we aimed to investigate the apoptotic and cytotoxic effects of different doses of cisplatin administered to colon cancer cells (HT29) for 72 h.Materials and Methods: Cells were treated with different (5-200¦ÌM) cisplatin concentrations for 72 hours. Apoptosis was determined using the EtBr- AO staining technique. The mRNA expression levels of BCL2L1, CASP3 and CASP8 were analyzed using the RT-PCR method.Results: Cisplatin had a cytotoxic effect in a dose dependent manner for a 72 h incubation period and the optimum apoptotic dose was found to be 50¦ÌM. We found a statistically significant increase in the mRNA levels of all three genes after the 5¦ÌM cisplatin treatment compared to control cells (p<0.05). 50¦ÌM cisplatin treatment did not lead to a significant difference in BCL2L1 mRNA levels (p>0.05) when compared to the control cells. However, CASP3 and CASP8 mRNA levels rose 2.2 and 3.0 fold respectively (p<0.05) after treatment with the same dose and for the same period.Conclusion: Our results showed that cisplatin effectively induced apoptosis via the caspase cascade signal pathway when it was administered to human colon cancer cells at 50¦ÌM for 72 h, and it suppressed the antiapoptotic pathway.
%K Apoptosis
%K HT29
%K Cell culture
%K Colon cancer
%K Cisplatin
%U http://mmj.dergisi.org/pdf.php3?id=600