%0 Journal Article
%T Hepatitis C virus E2 protein involve in insulin resistance through an impairment of Akt/PKB and GSK3¦Â signaling in hepatocytes
%A Ming-Ju Hsieh
%A Kuang-Ping Lan
%A Hao-Yu Liu
%A Xiao-Zong Zhang
%A Yaw-Feng Lin
%A Tzy-Yen Chen
%A Hui-Ling Chiou
%J BMC Gastroenterology
%D 2012
%I BioMed Central
%R 10.1186/1471-230x-12-74
%X Reverse transcription and real-time PCR, Western blot assay, Immunoprecipitation, Glucose uptake assay and analysis of cellular glycogen content.Results showed that E2 influenced on protein levels of insulin receptor substrate-1 (IRS-1) and impaired insulin-induced Ser308 phosphorylation of Akt/PKB and Ser9 phosphorylation of GSK3¦Â in Huh7 cells, leading to an inhibition of glucose uptake and glycogen synthesis, respectively, and eventually insulin resistance.Therefore, HCV E2 protein indeed involved in the pathogenesis of type 2 DM by inducing insulin resistance.
%K Hepatitis C virus
%K E2 envelope protein
%K Type 2 diabetes mellitus
%K Insulin resistance
%K Insulin receptor substrate-1 (IRS-1)
%K GSK3¦Â
%U http://www.biomedcentral.com/1471-230X/12/74/abstract