%0 Journal Article
%T Immobilization of glucoamylase on macroporous spheres
%A Milosavi£¿ Nenad B.
%A Prodanovi£¿ Radivoje M.
%A Jovanovi£¿ Slobodan M.
%A Vuj£¿i£¿ Zoran M.
%J Acta Periodica Technologica
%D 2004
%I Faculty of Technology, Novi Sad
%R 10.2298/apt0435207m
%X Glucoamylase was covalently immobilized through the spacer-arm of the poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) spheres by using a glutaraldehyde as a coupling agent. The influence of the enzyme load applied to the support on immobilization, yield and specific activity, has been determined. Obtained specific activity was 700 U/g with immobilization yield of 35 %. The Km value for immobilized glucoamylase was 1.28 % (w/v), pH and temperature optimum were 4.5 and 70¡ãC, respectively. The conversion of 20 % (w/w) starch hydrolysate achieved with the immobilized glucoamylase was 97 % after 5 hours.
%K Glucoamylase
%K immobilization
%K poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate)
%U http://www.doiserbia.nb.rs/img/doi/1450-7188/2004/1450-71880435207M.pdf