%0 Journal Article
%T The genetic characteristics of extended-spectrum ¦Â-lactamases (ESBLs) among Escherichia coli and Klebsiella pneumoniae isolates from urine specimens
%A Mirela Petra£¿cu
%A Mirela Flonta
%A Ariana M. Alma£¿
%J Human & Veterinary Medicine
%D 2011
%I Bioflux Society
%X Background & objectives: Extended spectrum ¦Â-lactamases (ESBLs) have emerged as a majorthreat worldwide with limited treatment options. The genotypes of ESBL producing strains largely remainunknown in Romania; hence the present study was aimed to determine the genetic characteristics of ESBLsin Escherichia coli and Klebsiella pneumonia isolates from urine specimens. Material and methods: Total152 consecutive non-duplicate clinical isolates of E. coli (n=70) and K. pneumoniae (n=82) collectedbetween January 2007 and April 2010 were isolated from urine samples in the Laboratory of InfectiousDiseases Hospital Cluj-Napoca. The isolates were examined phenotypically for ESBL production. ESBL strainswere further typed for the bla TEM/SHV/CTX-M/O XA genes by PCR using specific primers. Results: The TEM genewas detected in 77.63% (118 of 152) of the isolates followed by CTX-M (110 of 152 [73.36%]), OXA (101 of152 [66.44%]), and SHV (93 of 152 [61.18%]). From 70 E. coli isolates, 50 (71.42%) were positive for CTXMfollowed by OXA type (47; 67.14%), TEM (40; 57.14%) and SHV (23; 32.86%). From 82 K. pneumoniaeisolates, 78 (95.12%) were positive for TEM followed by SHV type (71; 86.58%), CTX-M (60; 73.17%) andOXA (53; 64.63%). Conclusions: The results of this study provide insights into the genetic characteristics ofESBLs among E. coli and K. pneumoniae isolates from urine specimens. The ESBL-positive E. coli isolatesinvestigated here encoded mainly CTX-Ms (71.42%), followed by OXA type (67.14%), TEM-type (57.14%),and SHV-type (32.86%) ¦Â-lactamases. The ESBL-positive K. pneumoniae isolates investigated here encodedmainly TEM type (95.12%) followed by SHV type (86.58%), CTX-M (73.17%) and OXA (64.63%).
%K Escherichia coli
%K Klebsiella pneumoniae
%K ESBL
%K CTX-M
%U http://www.hvm.bioflux.com.ro/docs/2011.3.58-65.pdf