%0 Journal Article
%T Clasificaci車n en subgrupos de cepas del virus sincitial respiratorio aisladas de un brote en Ciudad de La Habana
%A DANAY CHACON
%A ANGEL VALDIVIA
%A ANGEL GOYENECHEA
%A ISABEL OROPESA
%J Revista Cubana de Medicina Tropical
%D 1996
%I Centro Nacional de Informaci車n de Ciencias M谷dicas
%X Se detect車 un alto n迆mero de casos de enfermedades respiratorias agudas en ni os menores de 1 a o, ingresados en un hospital de Ciudad de La Habana. De 93 pacientes estudiados se obtuvieron 25 cepas del virus sincitial respiratorio. Los aislamientos virales fueron multiplicados en c谷lulas HEP-2, y despu谷s de observarse un efecto citop芍tico del 80 % se clasificaron en subgrupos por la t谷cnica de inmunofluorescencia indirecta, utilizando anticuerpos anti-prote赤na G del virus sincitial respiratorio. Todas las muestras estudiadas se clasificaron dentro del subgrupo A. Este tipo de estudio es el primero realizado en nuestro pa赤s, lo cual nos permiti車 profundizar en la causa viral de estas enfermedades y conocer que durante el brote circul車 el subgrupo A del virus sincitial respiratorio. A high number of acute respiratory diseases was detected among children under one year admitted in a hospital of Havana City. 25 respiratory syncytial virus strains were obtained from 93 patients studied. Viral isolations were multiplied in HEP-2 cells and after observing a cytopathic effect of 80 %, they were classified into subgroups by the indirect immunofluorescence technique, using anti-protein G antibodies from the respiratory syncytial virus. All the samples studied were classified within subgroup A. It is the first time a study like this is conducted in our country, which allowed us to deepen into the viral cause of these diseases and to know that the subgroup A of the respiratory syncytial virus circulated during the outbreak.
%K INFECCIONES DEL TRACTO RESPIRATORIO
%K TECNICA DEL ANTICUERPO FLUORESCENTE
%K VIRUS SINCITIAL RESPIRATORIO HUMANO
%K VIRUS SINCITIAL RESPIRATORIO HUMANO
%K RESPIRATORY TRACT INFECTIONS
%K FLUORESCENT ANTIBODY TECHNIQUE
%K RESPIRATORY SYNCYTIAL VIRUS
%K HUMAN
%K RESPIRATORY SYNCYTIAL VIRUS
%K HUMAN
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601996000200012