%0 Journal Article
%T Aplicaci車n de la secuenciaci車n nucleot赤dica de VP1 a la identificaci車n de Enterovirus humanos
%A Julio Antonio Barrios Olivera
%A Luis Sarmiento P谷rez
%A Odalys Vald谷s
%A Pedro Mas Lago
%J Revista Cubana de Medicina Tropical
%D 2003
%I Centro Nacional de Informaci車n de Ciencias M谷dicas
%X Se describi車 la introducci車n de un m谷todo molecular para la identificaci車n de los Enterovirus basado en la amplificaci車n, secuenciaci車n y an芍lisis filogen谷tico de la prote赤na VP1. Se demostr車 que este m谷todo reduce grandemente el tiempo requerido para la identificaci車n de los Enterovirus aislados y resulta de gran utilidad en la caracterizaci車n de aislamientos dif赤ciles de tipificar, con el empleo de los reactivos inmunol車gicos de rutina. Por la rapidez de ejecuci車n de la t谷cnica reviste vital importancia su uso durante epidemias, dada la r芍pida determinaci車n del agente causal. The introduction of a mollecular method to identify the Entoviruses based on the amplification, sequencing and phylogenetic analysis of protein VP1 was described. It was proved that this method reduces significantly the time required for the identification of the isolated Entoviruses and that it is very useful in the characterization of isolates which are difficult to typify by the routine immunoloigical reagents. As it is a very fast technqiue, its use is very important during epidemics to determine the causal agent rapidly.
%K INFECCIONES POR ENTEROVIRUS
%K MODELOS MOLECULARES
%K ENTEROVIRUS INFECTIONS
%K MODELS
%K MOLECULAR
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07602003000300001