%0 Journal Article
%T The Role of NO and cGMP in vasodilatory effect of 汕2-adrenoceptors in rat skin
%A Mojtaba Golmohammadi
%A Sohrab Hajizadeh
%A Mohammad Faghihi
%A Marzeieh Hosein
%J Physiology and Pharmacology
%D 2009
%I Iranian Society for Physiology and Pharmacology
%X Introduction: In recent study both endothelium-dependent and endothelium-independent mechanisms have been reported for the action of 汕-adrenoceptor. The aim of this study was to investigate on the role of nitric oxide (NO) and cyclic guanosin monophosphate (cGMP) in vasodilation mechanisms of 汕2-adrenoceptors (汕2-AR) in rat skin vessels. Methods: All drugs were injected subcutaneous into planar skin of hind paw. Injection volume was 10米l (5米l/min). Induction of anesthesia was perform with urethane 1.5 g/kg. Laser Doppler Flowmetery (LDF) technique was used for skin blood flow (SBF) monitoring. Results: The results obtained in this study showed that different doses of salbutamol, selective 汕2-AR agonist (1米M) caused a significant increase of SBF, but there was not any significant different in the response of different doses. Atenolol, selective 汕1-adrenoceptor antagonist (10米M) alone and with salbutamol had no significant effect on SBF. Propranolol, non selective 汕-adrenoceptor antagonist (1米M) by itself did not changed SBF, but significantly reduced the vasodilatory effect of salbutamol. LNNA, NO inhibitor (10米M) and methylen blue, cGMP inhibitor (3米M) caused a significant decrease of SBF 6/95% and 7/91% respectively. Salbutamol injection after LNNA and NO raised the SBF to 24/7% and 22.5% respectively, which shows a significant reduction in comparison to salbutamol＊s effect (42.73%). Conclusion: The results indicated that, salbutamol dilate rat skin vessels via 汕2- ARs. NO and cGMP involved in 汕2-ARs mediated vasodilation and contribute to regulation of vascular skin tone. To elucidate the exact mechanism of this response more studies are needed.
%K 2-adrenoceptor
%K skin blood flow
%K Salbutamol
%K Laser Doppler Flowmetery
%K Rat
%U http://www.phypha.ir/ppj/browse.php?a_code=A-10-175-112&slc_lang=en&sid=1