%0 Journal Article
%T EFFECT OF THE ORTHOSIPHON STAMINEUS, BENTH ON AMINOPYRINE METABOLISM IN RAT HEPATOCYTES
%A CHIN JIN HAN1
%A ABAS HJ. HUSSIN
%J Malaysian Journal of Pharmaceutical Sciences
%D 2007
%I 
%X One of the commonly used herbal medicines in Malaysia is Orthosiphon stamineus, Benth (family: Lamiaceae) or locally known as Misai Kucing. This experiment was undertaken to evaluate possible interaction of methanol extract of O. stamineus with aminopyrine, a model drug, in different age groups (young, adult and old) of Sprague-Dawley (SD) female rat hepatocytes. Hepatocytes were prepared by collagenase perfusion technique. Determination of aminopyrine N-demethylase activity was done by measuring formaldehyde formed. From these findings, only normal young female rat hepatocytes in the presence of 0.001 mg/ml of methanol extract of O. stamineus showed significant increase in aminopyrine N-demethylase activity. However, aminopyrine N-demethylase activity was not affected in hepatocytes of normal adult and old female SD rats. In conclusion, exposure of methanol extract of O. stamineus could affect phase I aminopyrine metabolism in normal young female SD rat hepatocytes but this effect was age-dependent.
%K Orthosiphon stamineus
%K Aminopyrine
%K Hepatocytes
%K Protein kinases
%U http://www.usm.my/mjps/MJPS%205%20(1)%202007/MJPS%205.1.4.pdf