%0 Journal Article
%T Optimization of Single Cell Protein Production by Candida utilis Using Juice Extracted from Pineapple Waste through Response Surface Methodology
%A Rosma
%A A.
%A Liong
%A M. T.
%A Mohd. Azemi
%A M. N.
%A Wan Nadiah
%A W. A.
%J Malaysian Journal of Microbiology
%D 2005
%I Malaysian Society for Microbiology
%X Response surface methodology was applied to optimize protein content in Candida utilis grown in pineapple waste medium. A three-level full factorial design was used to develop a quantitative interpretation of mathematical models between the two variables studied, inoculum size 2.0-10.0% (v/v) and total soluble solids in medium (1 -5  Brix) at 30 h fermentation time. Yeast cells were harvested, ruptured mechanically and the soluble extract was freeze-dried for determination of protein, vitamin-B, 5'-ribonucleotide and total sugar content. Maximum protein content in the yeast 66.61% (w/w) was obtained from the predicted optimum inoculum size of 7.83% (v/v) and Brix level of 3.02 . Highest level of biomass, vitamin-B, 5'-ribonucleotide and total sugar content within the experimental region increased 216.8%, 17.5%, 38.0% and 60.8% respectively after optimization. A verification experiment, conducted at optimized protein content conditions produced values that were close to the predicted values, indicating the reliability of the model used.
%K Response Surface Methodology
%K RSM
%K Yeast extract
%K Pineapple waste
%K Candida utilis
%U http://web.usm.my/mjm/issues/vol1no1/vol1_research2.pdf