%0 Journal Article %T QUANTITATIVE ANALYSIS OF RAW MATERIAL OF TETRACYCLINE AND ITS RELATED SUBSTANCES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY %A NABILA PERVEEN %A NAEEM HASAN KHAN %J Malaysian Journal of Pharmaceutical Sciences %D 2006 %I %X An isocratic method for the analysis of tetracycline (TC) by high performance liquid chromatography (HPLC) using polystyrene-divinylbenzene copolymer packing material is described. The method allows the complete separation of TC, 4-epitetracycline, anhydrotetracycline (ATC), 4-epianhydrotetracycline (EATC). A fermentation impurity, 2-acetyl-2-decarboxamidetetracycline is also resolved from TC. The mobile phase combines tert.-butanol, water and phosphate buffer, tetrabutylammonium sulphate and sodium ethylenediaminetetraacetate at pH 9.0 for elution at a temperature of 60ˇăC. Among the organic modifiers examined, only tetrahydrofuran and tert.-butanol gave good results. For practical reasons, tert.-butanol was retained as the final organic modifier. The preliminary experiments were carried out on polystyrene-divinylbenzene copolymer packing material (PRP-I and PLRP-S columns). The selectivity of the columns is comparable. PLRP-S column was chosen as it shows better separation of tetracycline ¨C anhydrotetracycline. The method was used to analyse the commercial samples. %K Tetracycline %K High performance liquid chromatography %K Polystyrene¨Cdivinylbenzene copolymer stationary phase %U http://www.usm.my/mjps/MJPS%204(2)%202006/MJPS%204.2.5.pdf