%0 Journal Article
%T EXPRESSION OF Bcl-2, Bcl-X, Bax AND p53 PROTEINS IN EPSTEIN BARR VIRUS POSITIVE AND NEGATIVE HODGKIN'S LYMPHOMA
%A Elmir Cickusic
%A Aida Arnautovic
%A Jasminka Mustedanagic-Mujanovic
%A Ermina Iljazovic
%J Acta Medica Saliniana
%D 2008
%I University Clinical Center Tuzla
%R 10.5457/ams.60.08
%X Introduction: Reed-Sternberg cells are typical for Hodgkin¡¯s lymphoma. They are transformed post-germinative B cells that not achieve successful immunoglobulin gene rearrangement, and therefore predetermined to apoptosis. Several mechanisms, including latent Epstein Barr virus infection (EBV), enable survival of Hodgkin¡¯s lymphoma neoplastic cells. Goal of this study is correlation of Hodgkin¡¯s lymphoma EBV status with expression of apoptosis associated proteins in neoplastic cells. Association of Hodgkin¡¯s lymphoma with EBV was determined by chromogenic hybridization in situ detection of EBER1 and EBER2. Material and Methods: Presence of apoptosis associated proteins: bcl-2, bcl-X, bax and p53, were determined immunohistochemicaly in neoplastic cells of 39 EBV positive, and 42 EBV negative Hodgkin¡¯s lymphomas. Results: Bcl-2 protein was detected in neoplastic cells in 22.2%, Bcl-X in 87.7%, Bax in 80.2% and p53 in 67.8% of cases. In 44.4% cases simultaneous presence of Bcl-X, Bax and p53 protein was detected. Bcl-X is significantly more produced in mixed cellularity, and Bcl-X and Bax in nodular sclerosis. No significant difference was found in production of all four proteins between EBV positive and negative Hodgkin¡¯s lymphoma. Median intensity of p53, Bcl-2, Bcl-X and Bax staining of complete examined group, EBV positive and EBV negative group show significant difference, except between Bcl-X and Bax. Discussion: Survival capacity of Hodgkin¡¯s lymphoma neoplastic cells depends on activity of numerous regulators of programmed cell death. Expression of bcl-2, bcl-X, bax and p53 proteins is not different in neoplastic cells of EBV positive and EBV negative Hodgkin¡¯s lymphoma.
%K Hodgkin¡¯s lymphoma
%K bcl-2
%K bcl-X
%K bax
%K p53
%K Epstein Barr virus
%U http://saliniana.com.ba/index.php?journal=ams&page=article&op=view&path%5B%5D=60