%0 Journal Article
%T Geographic distributions of Idh-1 alleles in a cricket are linked to differential enzyme kinetic performance across thermal environments
%A Diana L Huestis
%A Brenda Oppert
%A Jeremy L Marshall
%J BMC Evolutionary Biology
%D 2009
%I BioMed Central
%R 10.1186/1471-2148-9-113
%X We found significant GxE between temperature and three enzyme kinetic parameters, providing further evidence that the natural distributions of Idh-1 allele frequencies in A. socius are maintained by natural selection. Differences in enzyme kinetic activity across temperatures also mirror many of the geographic patterns observed in allele frequencies.This study further supports the hypothesis that the natural distribution of Idh-1 alleles in A. socius is driven by natural selection on differential enzymatic performance. This example is one of several which clearly document a functional basis for both the maintenance of common alleles and observed clines in allele frequencies, and provides further evidence for the non-neutrality of some allozyme alleles.Individuals within populations are under selection pressure to adapt to their environment; these adaptations can be morphological, physiological, or behavioral in nature. However, these diverse adaptations all have a molecular basis and the study of molecular adaptation to environmental conditions is an active area of research within evolutionary biology. One biochemical adaptation that lends itself to empirical study is the kinetic performance of different enzyme alleles (allozymes) under a range of environmental conditions, such as temperature. Allozyme alleles arise when a point mutation in the protein-coding sequence leads to an amino acid substitution which alters the charge, weight, and folding of the protein [1]. Amino acid substitutions may also affect the function of the protein, altering optimal ranges for temperature, pH, or substrate concentration. Most amino acid changes will likely be deleterious and quickly eliminated by purifying selection [2,3]. However, some substitutions may not significantly affect the function of the protein, and are therefore selectively neutral, while others may improve enzyme function and be favored by selection.Originally, evolutionary biologists and geneticists thought genetic
%U http://www.biomedcentral.com/1471-2148/9/113