%0 Journal Article %T Diagn車stico morfol車gico de Helicobacter pylori mediante citolog赤a g芍strica por cepillado %A Sara Mandado P谷rez %A Bienvenido Gra Oramas %A Miguel Gonz芍lez Carvajal %A Manuel Paniagua Est谷vez %J Revista Cubana de Medicina %D 2003 %I ECIMED %X Se conoce que en Cuba la citolog赤a g芍strica por cepillado no hab赤a sido utilizada para el diagn車stico morfol車gico del Helicobacter pylori. Se realiz車 en el Instituto de Gastroenterolog赤a, mediante endoscopia, a 625 pacientes adultos, 585 con gastritis cr車nica y 40 con 迆lcera g芍strica. Se fijaron las muestras en alcohol de 95∼ Se probaron las coloraciones de Papanicolaou, hematoxilina y eosina, Gram, safranina y azul de metileno al 2 %. Se observaron las muestras en un microscopio 車ptico previamente calibrado. Se diagnostic車 H. pylori en 310 gastritis cr車nicas y en 28 迆lceras, 2 de las cuales eran malignas. Se diagnostic車 H. heilmannii en un caso con 迆lcera g芍strica negativo de H. pylori. Se eligi車 el azul de metileno al 2 % para la coloraci車n. It is known that in Cuba the gastric brush citology has not been used for the morphological diagnosis of Helicobacter pylori. It was performed by endoscopy to 625 adult patients , 585 with chronic gastritis and 40 with stomach ulcer, at the Institute of Gastroenterology. The samples were fixed in alcohol 95 %. Papanicolaou*s stains, hematoxylin and eosin , Gram*s stain, safranin and methylene blue were tested. The samples were observed on a previously calibrated optical microscope. H. pylori was diagnosed in 310 chronic gastritis and in 28 ulcers, 2 of them were malignant. H. heilmannii was diagnosed in a case with stomach ulcer negative to H. pylori. Methylene blue 2 % was selected for staining. %K HELICOBACTER PYLORI %K HELICOBACTER PYLORI %K GASTRITIS CRONICA %K ULCERA GASTRICA %K ENDOSCOPIA DEL SISTEMA DIGESTIVO %K COLORACION %K HELICOBACTER PYLORI %K HELICOBACTER PYLORI %K GASTRITIS %K STOMACH ULCER %K ENDOSCOPY %K DIGESTIVE SYSTEM %K STAINING %U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034-75232003000100004