%0 Journal Article
%T Research: In-vitro CULTURE ESTABLISHMENT AND SHOOT INDUCTION OF Mallotus philippenesis (Lam.) M. ARG
%A Jaya Sharma*
%A Shagufta Khan
%A Ranjana Varma
%J Pharmacie Globale : International Journal of Comprehensive Pharmacy
%D 2012
%I 
%X This research work has given a method for in-vitro propagation by developed seedling than shoot induction in M. philippenesis (lam.) M. Arg. which is a medicinal plant of high commercial value. For shoot formation both apical shoot buds & meristems were used. This was achieved on MS medium containing BAP in combination with NAA & Kinetin alone or in combination with BAP was tested. Best shoot initiation response was achieved on MS Medium supplemented with 6.0 mg/l BAP + 1.0 mg/l NAA on both apical shoot bud & meristem explants. The maximum percentage of explants (meristems & apical shoot bud) forming shoots are 85 ¡À 0.23 and 95 ¡À 0.23 respectively. Increase or decrease in concentration of BAP 6.0 mg/l + NAA 1.0 mg/l, increases, no. of days required for shoot initiation. Meristems showed more pronounced effect of shoot formation than apical shoot bud explants. These induced shoots were increase in their number & in size when were given subsequent incubation period. Addition of BAP in combination of Kn or Kn alone failed to show good shoot initiation response. The shoot induction protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite medicinal plant. Further work for standardization of efficient in-vitro protocol for best shoot multiplication & in-vitro rooting is under progress in our laboratory.
%K M. philippenesis
%K 6-Benzyllaminopurine (BAP)
%K Naphthalene acetic acid (NAA)
%K 6-Furfurylamino purine (kinetin
%K KN)
%K Murashige and Skoog medium (MS)
%K medicinal plant conservation
%U http://pharmacie-globale.info/index.php?option=com_docman&task=doc_view&gid=247&tmpl=component&format=raw&Itemid=41