%0 Journal Article
%T Application of Liquid Chromatography with Diode-Array Detector for Determination of Acetamiprid and 6-chloronicotinic Acid Residues in Sweet Cherry Samples
%A Sanja Lazi£¿
%A Dragana £¿unjka
%A Nada Grahovac
%A Val¨¦ria Guzsv¨¢ny
%J Pesticidi i Fitomedicina
%D 2012
%I Institute of Pesticides and Environmental Protection
%X rapid and simple method for simultaneous determination of acetamiprid and itsmetabolite 6-chloronicotinic acid in sweet cherry samples has been developed. This residueanalysis method is based on the reversed phase separation on C18 column with gradientelution. Analytes¡¯ determination and quantification were performed by high performanceliquid chromatography (HPLC) with diode-array detector and chromatogramswere extracted at 230 nm. Extraction efficiency experiments demonstrated the ability ofthis method to extract neonicotinoids from sweet cherry samples. These insecticides wereextracted with a mixture of acetonitril/0.1N ammonium-chloride (8/2, v/v). The averagerecoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in therange of 95-101% and 73-83%, respectively, with the associated relative standard deviations(RSDs) <5%. Expanded measurement uncertainties for the analyzed compounds were 2.7and 3.01%. The limit of quantification (LOQ) was 10 ¦Ìg/kg and 30 ¦Ìg/kg for acetamiprid and6-chloronicotinic acid, respectively. Thus, the developed HPLC/DAD method can be considereda useful tool for sensitive and rapid determination of acetamiprid and 6-chloronicotinicacid. Hence, the method may find further application in the analysis of real sweet cherrysamples contaminated with these insecticides at a ppb level.
%K Acetamiprid
%K 6-chloronicotinic acid
%K HPLC/DAD
%K Sweet cherry
%K Pesticide residues
%U http://www.pesting.org.rs/download.php/documents/27-4/27-4%20321-329.pdf