%0 Journal Article
%T Obtenci車n y procesamiento de progenitores hematopoy谷ticos de sangre perif谷rica para terapia celular en enfermedades angiol車gicas Obtaining and processing of peripheral blood hematopoietic progenitor cells for cell therapy of angiological diseases
%A Ana Iris Gonz芍lez Iglesias
%A Mariela Forrellat Barrios
%A Tania Gonz芍lez Su芍rez
%A Odalis Salgado Arozena
%J Revista Cubana de Hematologˋ-a, Inmunologˋ-a y Hemoterapia
%D 2011
%I Editorial Ciencias M谷dicas
%X Se evalu車 la seguridad y efectividad de un m谷todo manual de recolecci車n de c谷lulas progenitoras hematopoy谷ticas de sangre perif谷rica movilizadas con factores estimuladores de colonias granuloc赤ticas (FEC-G) de producci車n nacional (Leukocim y Hebervital). Se estudiaron 250 pacientes seleccionados para terapia celular en el Servicio de Angiolog赤a del Hospital General Docente "Enrique Cabrera". La obtenci車n y separaci車n de las c谷lulas mononucleares aut車logas de sangre perif谷rica (CMN-SP) se realiz車 mediante el m谷todo dise ado en el Instituto de Hematolog赤a e Inmunolog赤a. Para valorar la eficacia del m谷todo se analizaron en el concentrado obtenido las variables: contenido de c谷lulas nucleadas, de c谷lulas mononucleadas y de c谷lulas CD 34+. Adem芍s, se determin車 la viabilidad celular y la contaminaci車n microbiol車gica. Se comprob車 la eficiencia y seguridad del m谷todo de recolecci車n y procesamiento para la obtenci車n de un concentrado con un contenido de c谷lulas mononucleares adecuado, sin complicaciones de importancia cl赤nica. Se demostr車 la eficacia de los factores estimuladores de colonias granuloc赤ticas empleados. Los efectos adversos de la movilizaci車n resultaron ligeros e independientes del factor estimulador utilizado. The safety and effectiveness of a manual collection method of peripheral blood hematopoietic progenitor cells mobilized by the Cuban-made granulocytic colony-stimulating factors (Leukocim and Hebervital) were evaluated. Two hundred patients, who had been selected for the cell therapy at the Angiology Service of "Enrique Cabrera" General Teaching Hospital, were studied. The method designed by the Institute of Hematology and Immunology served to obtain and separate autologous mononuclear cells from the peripheral blood. For the purpose of assessing the efficacy of this method, the variables contents of nucleate cells, of mononucleate cells and of CD 34+ cells were analyzed in the final concentrate. Additionally, the cell viability and the microbiological pollution were determined. The efficiency and safety of the collecting and processing method for obtaining one concentrate with adequate content of mononuclear cells and no significant clinical complications was confirmed. The efficacy of the Cuban granulocytic colony-stimulating factors was proved. The adverse effects of the mobilization were mild and unrelated to the used stimulating factor.
%K medicina regenerativa
%K c谷lulas madre
%K factor estimulador de colonias granuloc赤ticas
%K c谷lulas mononucleares
%K regenerative medicine
%K stem cells
%K granulocytic colony-stimulating factors
%K mononuclear cells
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0864-02892011000300012