%0 Journal Article
%T Coordinated gene expression during gilthead sea bream skeletogenesis and its disruption by nutritional hypervitaminosis A
%A Ignacio Fern¨˘ndez
%A Maria Darias
%A Karl B Andree
%A David Mazurais
%A Jose Zambonino-Infante
%A Enric Gisbert
%J BMC Developmental Biology
%D 2011
%I BioMed Central
%R 10.1186/1471-213x-11-7
%X During gilthead sea bream larval development, specific expression profiles for each gene were tightly regulated during fish morphogenesis and correlated with specific morphogenetic events and tissue development. Dietary hypervitaminosis A during early larval development disrupted the normal gene expression profile for genes involved in RA signalling (RARA), VA homeostasis (RBP) and several genes encoding ECM proteins that are linked to skeletogenesis, such as bglap and mgp.Present data reflects the specific gene expression patterns of several genes involved in larval fish RA signalling and skeletogenesis; and how specific gene disruption induced by a nutritional VA imbalance underlie the skeletal deformities. Our results are of basic interest for fish VA signalling and point out some of the potential molecular players involved in fish skeletogenesis. Increased incidences of skeletal deformities in gilthead sea bream fed with hypervitaminosis A were the likely ultimate consequence of specific gene expression disruption at critical development stages.Skeletogenesis is a critical process in vertebrates during which the skeleton develops in a genetically programmed manner, leading to normal anatomy that provides support and protection for the internal organs. In mammals, this process includes the differentiation and proliferation of different cell types, such as chondrocytes, osteoblasts, osteocytes and osteoclasts, which determine the size, shape and mineral composition of bone structures. The expression of specific genes deeply underlies these processes of cell proliferation and differentiation, which are also controlled by biotic and abiotic factors as well as individual genetic characteristics. Thus, determining factors and conditions that control and perturb those processes at the transcriptional level could be useful for deciphering the specific mechanisms behind these processes.Teleost fish are considered to be the first vertebrate group to develop a bony skeleto
%U http://www.biomedcentral.com/1471-213X/11/7