%0 Journal Article
%T Morphometric Investigation of Neurons in the Hippocampal CA1, CA3 Areas and Dentate Gyrus in a Rat Model of Sepsis Investigaci車n Morfom谷trica de Neuronas en las 芍reas Hipocampales CA1, CA3 y Giro Dentado en un Modelo de Sepsis en Rata
%A Ilker M Kafa
%A Ilknur Ari
%A M. Ayberk Kurt
%J International Journal of Morphology
%D 2010
%I Sociedad Chilena de Anatom赤a
%X Approximately, half of the patients with progressive sepsis develop encephalopathy, but there is scarce knowledge onto question that how the sepsis associated encephalopathy contributes brain dysfunction. Hippocampus is one of the most vulnerable regions during experimental sepsis. In the present study, effects of sepsis on the neuronal density and morphology in CA1, CA3 and DG areas were investigated in a rat model of intraperitoneal sepsis. Twenty-four Wistar rats were divided into three different groups: faecal peritonitis group, sham-operated and un-operated control groups. Pyramidal neuron volume density was significantly higher in CA1 area of the faecal peritonitis group compared to both un-operated (p<0.05) and sham-operated (p<0.05) groups. Pyramidal neuron volume density was also significantly higher in CA3 area of the faecal peritonitis group compared to both un-operated (p<0.05) and sham-operated (p<0.05) groups. Mean nuclear diameter of pyramidal neurons in CA1 area of the faecal peritonitis group was significantly lower (p<0.05) compared to un-operated control group. Dark, shrunken neurons were frequently observed and neuroglial cells appeared to be prevalent in the faecal peritonitis group compared to control groups. These results collectively suggest that intraperitoneal sepsis does not initiate cell death in the early stages of sepsis, although morphological signs of neurodegeneration start to appear. Aproximadamente, la mitad de los pacientes con sepsis progresiva desarrollan encefalopat赤a, pero hay escaso conocimiento en cuesti車n de como la sepsis asociada con encefalopat赤a contribuye a la disfunci車n cerebral. El hipocampo es una de las regiones m芍s vulnerables durante la sepsis experimental. En el presente estudio, fueron analizados los efectos de la sepsis sobre la densidad neuronal y la morfolog赤a en las 芍reas CA1, CA3 y giro dentado en un modelo de sepsis intraperitoneal en rata. Veinticuatro ratas Wistar se dividieron en tres grupos diferentes: grupo de peritonitis fecal, operaci車n simulada y control no operado. La densidad del vol迆men piramidal fue significativamente mayor en el 芍rea CA1 del grupo con peritonitis fecal en comparaci車n con los grupos no operados (p<0,05) y la operaci車n simulada (p<0,05). La densidad de volumen de las neuronas piramidales fue significativamente mayor en el 芍rea CA3 del grupo peritonitis fecal en comparaci車n con los no operado (p<0,05) y la operaci車n simulada (p<0,05). El di芍metro promedio nuclear de las neuronas piramidales en la zona CA1 del grupo de peritonitis fecal fue significativamente menor (
%K Sepsis
%K Modelos de sepsis animal
%K Peritonitis fecal
%K Hipocampo
%K Sepsis asociada a encefalopat赤a
%K Sepsis
%K Animal models of sepsis
%K Faecal peritonitis
%K Hippocampus
%K Sepsis associated encephalopathy
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100026