%0 Journal Article
%T Intestinal Wall Atrophy and Increase of Sulphomucin Secretion in the Jejunal Epithelium of Rats Submitted to Severe Protein Malnutrition Atrofia de la Pared Intestinal y Aumento de la Secreci¨®n de Sulfomucinas en el Epit¨¦lio Yeyunal de Ratas Sometidas a Intensa Desnutrici¨®n Prot¨¦ica
%A C¨¢ssia Luiza de Marins Franco
%A D¨¦bora de Mello Gon£¿ales Sant'Ana
%A Eduardo Jos¨¦ de Almeida Ara¨²jo
%J International Journal of Morphology
%D 2010
%I Sociedad Chilena de Anatom¨ªa
%X The effects of severe protein malnutrition on the morphometry of the intestinal wall in the jejunum of adult rats were evaluated in this study. Six 90-day-old Wistar rats (Rattus norvegicus) were assigned into two groups: CG (Control Group, n = 3) and MG (Malnourished Group, n = 3). CG animals were fed a 26%-protein chow whereas controls were fed a 4%-protein chow. After 90 days, part of the jejunum was collected and subjected to routine histology. HE-staining and histochemical techniques - Periodic Acid Schiff (PAS) and Alcian Blue (AB), pH 2.5/pH 1.0 - were used for the detection of glycoconjugates in 4¦Ìm -thick transverse cuts. Morphometric analysis of the HE-stained cuts revealed a decrease of the total thickness of the jejunal wall, mostly on the following layers: external muscle and mucosa - enterocyte height also decreased. Cuts stained by using histochemical techniques for the detection of glycoconjugates revealed maintenance of goblet cells reactive to PAS and AB pH 2.5, whereas the number of cells reactive to AB pH 1.0 increased. Protein malnutrition was concluded to provoke severe atrophy of the jejunal wall and an increase of sulphomucin secretion within the intestinal epithelium. Fueron evaluados los efectos de la desnutrici¨®n proteica severa sobre la morfometr¨ªa de la pared intestinal del yeyuno de ratas adultas . Para esto, se utilizaron seis ratas (Rattus norvegicus) Wistar, con 90 d¨ªas de edad, distribuidas en dos grupos: GC (grupo control, n=3) y el GD (grupo desnutrido, n=3). Los animales del GC recibieron raci¨®n con un contenido proteico de 26% y los del GD raci¨®n con 4% de prote¨ªnas. Despu¨¦s de 90 d¨ªas de experimentaci¨®n, parte del yeyuno fue retirado y sometido a un procesamiento histol¨®gico de rutina. Cortes transversales de 4 ¦Ìm fueron te idos con HE y t¨¦cnicas histoqu¨ªmicas (Periodic Acid Schifff (PAS) y Alcian Blue (AB) pH 2,5 y pH 1,0.) para evidenciar glicoconjugados. El an¨¢lisis morfom¨¦trico te idos con HE demostr¨® reducci¨®n en el grosor total de la pared del yeyuno, especialmente de las t¨²nicas muscular y mucosa, adem¨¢s se observ¨® una disminuci¨®n en la altura de los entericitos. Los cortes te idos con t¨¦cnicas histoqu¨ªmicas revelaron que el n¨²mero de c¨¦lulas caliciformes reactivas al PAS y al AB pH 2,5 se mantuvo; por otro lado, hubo un aumento en el n¨²mero de c¨¦lulas reactivas al AB pH 1,0. Se concluye que la desnutrici¨®n proteica severa provoca atrofia de la pared yeyunal y aumento de la secreci¨®n de sulfomucinas en el epitelio intestinal.
%K Desnutrici¨®n proteico-energ¨¦tica
%K Intestino delgado
%K Morfometr¨ªa
%K Mucinas
%K Protein-energetic malnutrition
%K Small intestine
%K Morphometry
%K Mucins
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000200026