%0 Journal Article
%T Fertilization in C. elegans requires an intact C-terminal RING finger in sperm protein SPE-42
%A Luke D Wilson
%A Jacqueline M Sackett
%A Bryce D Mieczkowski
%A Abigail L Richie
%A Kara Thoemke
%A Jon N Rumbley
%A Tim L Kroft
%J BMC Developmental Biology
%D 2011
%I BioMed Central
%R 10.1186/1471-213x-11-10
%X We made an in silico structural model of the SPE-42 RING finger domain based on primary sequence analysis and previously reported RING structures. To test the model, we created spe-42 transgenes coding for mutations in each of the 8 cysteine residues predicted to coordinate Zn++ ions in the RING finger motif. Transgenes were crossed into a spe-42 null background and protein function was measured by counting progeny. We found that all 8 cysteines are required for protein function. We also showed that sequence differences between the C-terminal 29 and 30 amino acids in C. elegans and C. briggsae SPE-42 following the RING finger domain are not responsible for the failure of the C. briggsae SPE-42 homolog to rescue C. elegans spe-42 mutants.The results suggest that a bona fide RING domain is present at the C-terminus of the SPE-42 protein and that this motif is required for sperm-egg interactions during C. elegans fertilization. Our structural model of the RING domain provides a starting point for further structure-function analysis of this critical region of the protein. The C-terminal domain swap experiment suggests that the incompatibility between the C. elegans and C. briggsae SPE-42 proteins is caused by small amino acid differences outside the C-terminal domain.The union of sperm and egg pronuclei to form a new organism is the endpoint of a carefully choreographed process that includes gamete recognition, binding, and fusion of plasma membranes. Our understanding of the molecular processes underlying these phenomena has been shaped by studies in sea urchins [1], Chlamydomonas [2-4], Drosophila [5], mice [6-8] and, within the last decade, the nematode Caenorhabditis elegans [9-12]. C. elegans is particularly suited to the discovery of molecules necessary for spermatogenesis or fertilization because its hermaphroditic mode of reproduction is unique among genetic model organisms. Spermatogenesis defective (spe) mutants discovered in genetic screens using hermaphrodit
%U http://www.biomedcentral.com/1471-213X/11/10