%0 Journal Article %T Expression of Death Cellular Receptors FAS/CD95 and DR4 During Porcine Placentation Expresi車n de los Receptores de Muerte Celular FAS/CD95 y DR4 Durante la Placentaci車n Porcina %A C Merkis %A A Cristofolini %A E Sanchis %A M Koncurat %J International Journal of Morphology %D 2010 %I Sociedad Chilena de Anatom赤a %X Apoptosis is a permanent and dynamic physiological process by which an organism eliminates the undesirable cells without causing an inflammatory response. The objective of this work was to study the expression of FAS, DR4 and other members of the TNF-R1 superfamily extrinsic route apoptotic receptors the DNA fragmentation and the cellular apoptosis in placental samples at the early, mid and late pregnancy on ㊣ 30, ㊣ 55 and ㊣ 114 gestational days, respectively. We used placental histological sections of samples fixed in buffered saline formaldehyde. Immunohistochemical techniques were performed to detect the apoptotic receptors, whereas the DNA fragmentation was detected by TUNEL reaction and apoptotic cellular ultrastructure was detected by TEM conventional techniques. Apoptosis related receptors were immunolocalized in the early pig gestation and correlated with apoptosis, suggesting a role in the cellular remodelling of the placenta. At gestation day 55, apoptosis might be correlated to FAS route, but not by DR4-mediating pathway. At the end of gestation, increased apoptosis and both receptors markers were detected showing cellular death due to the extrinsic route through FAS and DR4 receptors. In conclusion, the immunolocalization of FAS and TNF R-1 receptors along the pig placental development correlates with TUNEL reaction and with apoptotic ultrastructure observed by TEM and seems to occur through different pathways along gestation. La apoptosis es un proceso fisiol車gico, din芍mico y permanente a trav谷s del cual un organismo elimina c谷lulas indeseables sin provocar una respuesta inflamatoria. El objetivo del presente trabajo fue estudiar la expresi車n de los receptores de la v赤a extr赤nseca de apoptosis, FAS, DR4 y otros miembros de la superfamilia TNF-R1, la fragmentaci車n del ADN y la apoptosis celular a trav谷s de TEM, en muestras placentarias del inicio, la mitad y el final de la gestaci車n, hacia el d赤a ㊣ 30, ㊣ 55 y ㊣ 114 de pre ez, respectivamente. Se realizaron cortes histol車gicos de las muestras placentarias fijadas en formol tamponado. Para la detecci車n de los receptores de apoptosis se realizaron t谷cnicas inmunohistoqu赤micas, para el estudio de la fragmentaci車n del ADN se utiliz車 el ensayo TUNEL y para el an芍lisis de la ultraestructura celular apopt車tica la t谷cnica convencional de TEM. La inmunolocalizaci車n de los receptores de muerte celular al inicio de la pre ez porcina sugiere el rol de la apoptosis en la remodelaci車n celular placentaria. Hacia el d赤a 55 de pre ez, la apoptosis detectada ocurrir赤a 迆nicamente a trav谷s de la v赤a del receptor %K FAS/CD95 %K DR4 y superfamilia TNF-R1 %K Receptors de apoptosis %K FAS/CD95 %K DR4 and TNF-R1 family %K Apoptosis receptors %K Pig placenta %K Placenta porcina %U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000300026