%0 Journal Article
%T Effects of Zinc During Lactation on the Junctional Epithelium and Inserted Gum in Rats Efectos del Zinc Durante la Lactancia sobre el Epitelio de Uni¨®n y la Enc¨ªa Insertada en Ratas
%A Ana Luiza de Carvalho Felippini
%A Miguel Angel Sala
%A Ruberval Armando Lopes
%A Gilberto Andr¨¦ e Silva
%J International Journal of Morphology
%D 2013
%I Sociedad Chilena de Anatom¨ªa
%X The purpose of this study was to evaluate the effects of zinc, during lactation, on the junctional epithelium and inserted gum of the first upper molar of rats. The study used one-day old male rats, divided into two groups: those whose mother had been treated with 300 mg zinc chloride (ZnCl2) in the drinker water (treated group), and those whose mothers did not receive ZnCl2 (control group). After 21 days, the rat pups were sacrificed. Using karyometrical techniques, the greater (D) and smaller (d) nuclear diameters of the different layers of the junctional and inserted gum epithelia were determined, and the mean geometric diameter, D/d ratio, perimeter, area, volume, volume/area ratio, eccentricity, shape coefficient, and the contour index were estimated. The 100-point Merz grid was used with the purpose of evaluating the citoplasmatic and celular volume, the nucleus/citoplasm relationship, number density, outer surface/basal layer ratio, the thickness of epithelial layers, and the surface density. The results were submitted to statistical analysis using the Wilcoxon-Mann-Whitney test. The nuclei of the studied structures were significantly smaller, and the stereological results demonstrated that there were smaller cells, hence meaning a greater number of cells per mm3 of tissue, in the treated group. Zinc caused changes on the studied epitheliums, according to morphometric and stereological evaluations. El objetivo de este estudio fue evaluar los efectos del zinc durante la lactancia, sobre el epitelio de uni¨®n y la enc¨ªa insertada del primer molar superior de ratas. Fueron utilizadas ratas macho de un d¨ªa de edad, divididas en dos grupos: aquellas cuyas madres hab¨ªan sido tratadas con 300 mg de cloruro de zinc (ZnCl2) con agua del bebedero (grupo tratado) y aquellas cuyas madres no recibieron ZnCl2 (grupo control). Las cr¨ªas fueron sacrificadas despu¨¦s de 21 d¨ªas. Utilizando t¨¦cnicas cariom¨¦tricas fueron medidos los di¨¢metros mayor (D) y menor (d) de los n¨²cleos de las c¨¦lulas de los diferentes estratos del epitelio de uni¨®n y de la enc¨ªa insertada, estim¨¢ndose el di¨¢metro geom¨¦trico medio, la relaci¨®n D/d, per¨ªmetro, ¨¢rea, volumen, relaci¨®n volumen/¨¢rea, excentricidad, coeficiente de forma e ¨ªndice de contorno. Fue usada la rejilla de Merz, de 100 puntos, con la finalidad de evaluar el volumen celular y citoplasm¨¢tico, la relaci¨®n n¨²cleo/citoplasma, densidad num¨¦rica, relaci¨®n superficie externa/superficie basal, espesor de las capas epiteliales y densidad de superficie. Los resultados fueron sometidos a an¨¢lisis estad¨ªstico mediante el test de Wilc
%K Zinc
%K Lactancia
%K Epitelio de uni¨®n
%K Enc¨ªa insertada
%K Zinc
%K Lactation
%K Junctional epithelium
%K Inserted gum
%U http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100024