%0 Journal Article
%T Effects of D-002 on aspirin-induced ulcers and neutrophil infiltration on the gastric mucosa Efecto del D-002 sobre la ¨²lcera inducida por aspirina asociada al infiltrado de neutr¨®filos en la mucosa g¨¢strica
%A Maikel Valle Clara
%A Miriam Noa Puig
%A Sarah¨ª Mendoza Casta£¿o
%A Ambar Oyarz¨¢bal Yera
%J Revista Cubana de Farmacia
%D 2012
%I ECIMED
%X Introduction: the gastric mucosa is susceptible to the effects of aggressive factors, which are counterbalanced by mucosal defensive factors. Acid peptic diseases result from the imbalance between these aggressive and defensive factors. Aspirin-induced ulcer is a model of NSAIDs-induced damage in which neutrophil infiltration plays a key role. Objective: this paper investigates the protective effect of D-002 against aspirin-induced ulcers and associated neutrophil infiltration in the gastric mucosa. Methods: rats were randomized into six groups of 8 rats each. A negative vehicle control, and five aspirin (300 mg/kg)-treated groups: a positive control, orally treated with the vehicle, three with D-002 (25, 50 and 100 mg/kg, respectively) and other with 10 mg/kg Omeprazole. Five hours after induced damage the rats were sacrificed. The stomachs were removed and opened, and lesions examined macroscopically and microscopically. Ulcer indexes and neutrophil infiltration per ulcer areas were measured. Results: all positive, none negative, controls exhibited aspirin-induced ulcers. Oral treatment with D-002 (25-100 mg/kg) dose-dependently and significantly reduced aspirin-induced gastric lesions (37 to 75 %), the mean number of microscopic ulcers (40 to 72 %) and neutrophil infiltration (41.7 to 83.1 %) in the rat gastric mucosa. Conclusion: Oral treatment with D-002 (25-100 mg/kg) effectively protects against aspirin-induced ulcers and decreases the neutrophil infiltration in the gastric mucosa induced by aspirin ulceration. Introducci¨®n: la integridad de la mucosa g¨¢strica depende del balance entre los factores agresivos y defensivos. La ¨²lcera inducida por aspirina es un modelo de da o por antiinflamatorios no esteroidales en el cual el infiltrado de neutr¨®filos desempe a una funci¨®n fundamental. Objetivo: evaluar el efecto protector del D-002 sobre la ¨²lcera inducida por aspirina asociada al infiltrado de neutr¨®filos en la mucosa g¨¢strica. M¨¦todos: las ratas fueron aleatorizadas en seis grupos de ocho animales cada uno. Un control negativo con veh¨ªculo y cinco grupos tratados con aspirina (300 mg/kg): un control positivo, tratado por v¨ªa oral con veh¨ªculo, tres grupos con D-002 (25, 50 and 100 mg/kg respectivamente) y otro con omeprazol 10 mg/kg. Cinco horas despu¨¦s de inducido el da o las ratas fueron sacrificadas y se extrajeron sus est¨®magos para su an¨¢lisis morfol¨®gico. Se determin¨® el ¨ªndice de ¨²lcera, el n¨²mero de ¨²lceras microsc¨®picas y el n¨²mero de neutr¨®filos por ¨¢rea ulcerada. Resultados: todos los controles positivos y ninguno negativo mostraron
%K D-002
%K ¨²lcera g¨¢strica
%K infiltraci¨®n de neutr¨®filos
%K aspirina
%K ratas
%K D-002
%K gastric ulcer
%K neutrophil infiltration
%K ASA
%K rats
%U http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0034-75152012000200012