%0 Journal Article
%T Comparison of haematological parameters determined by the Sysmex KX - 2IN automated haematology analyzer and the manual counts
%A Samuel O Ike
%A Thomas Nubila
%A Ernest O Ukaejiofo
%A Imelda N Nubila
%A Elvis N Shu
%A Ifeyinwa Ezema
%J BMC Clinical Pathology
%D 2010
%I BioMed Central
%R 10.1186/1472-6890-10-3
%X Sixty (60) subjects were randomly selected from both apparently healthy subjects and those who have different blood disorders from the University of Teaching Hospital (UNTH), Ituku-Ozalla, Enugu, Enugu State, Nigeria. Three (3)mls of venous blood sample was collected aseptically from each subject into tri-potassium ethylenediamine tetra-acetic acid (K3EDTA) for the analysis of haematological parameters using the automated and the manual methods.The blood film report by the manual method showed that 50% of the subjects were normocytic-normochromic while the other 50% revealed different abnormal blood pictures. Also, there were statistically significant differences (p < 0.05) in mean cell hemoglobin concentrations (MCHC) between the two methods. Similarly, the mean (S.E) values of hemoglobin, packed cell volume, platelet and total white cell counts demonstrated statistically significant difference (p < 0.001) and correlated positively when both methods were compared.From the present study, it can be concluded that the automated hematology analyzer readings correlated well with readings by the standard manual method, although the latter method gave additional diagnostic information on the blood pictures. While patients' care and laboratory operations could be optimized by using manual microscopic examination as a reflective substitute for automated methods, usage of automated method would ease our workload and save time for patients.The automated hematology analyzer with complete blood count (CBC) results has replaced the traditional manual or individual assay methods for haematological parameters and the eye count leucocyte differential as the initial screening and detection system for haematological abnormalities in modern hospitals and clinics[1]. The traditional review of all automated hematology instrument results by preparation, staining and microscopic examination of a blood film examination has disappeared in most institutions[2]. The reasons are the more accur
%U http://www.biomedcentral.com/1472-6890/10/3