%0 Journal Article
%T Ingredients of Huangqi decoction slow biliary fibrosis progression by inhibiting the activation of the transforming growth factor-beta signaling pathway
%A Jin-Xing Du
%A Ming-Yu Sun
%A Guang-Li Du
%A Feng-hua Li
%A Cheng Liu
%A Yong-Ping Mu
%A Gao-Feng Chen
%A Ai-Hua Long
%A Yan-Qin Bian
%A Jia Liu
%A Cheng-Hai Liu
%A Yi-Yang Hu
%A Lie-Ming Xu
%A Ping Liu
%J BMC Complementary and Alternative Medicine
%D 2012
%I BioMed Central
%R 10.1186/1472-6882-12-33
%X A liver fibrosis model was induced by ligation of the common bile duct (BDL) in rats. Sham-operation was performed in control rats. The BDL rats were randomly divided into two groups: the BDL group and the IHQD group. IHQD was administrated intragastrically for 4 weeks. At the end of the fifth week after BDL, animals were sacrificed for sampling of blood serum and liver tissue. The effect of IHQD on the TGF¦Â1 signaling pathway was evaluated by western blotting and laser confocal microscopy.Decreased content of hepatic hydroxyproline and improved liver function and histopathology were observed in IHQD rats. Hepatocytes, cholangiocytes, and myofibroblasts in the cholestatic liver injury released TGF¦Â1, and activated TGF¦Â1 receptors can accelerate liver fibrosis. IHQD markedly inhibited the protein expression of TGF¦Â1, TGF¦Â1 receptors, Smad3, and p-ERK1/2 expression with no change of Smad7 expression.IHQD exert significant therapeutic effects on BDL-induced fibrosis in rats through inhibition of the activation of TGF¦Â1-Smad3 and TGF¦Â1-ERK1/2 signaling pathways.
%K Ingredients of Huangqi decoction
%K Cholestatic liver fibrosis
%K Transforming growth factor beta 1
%K Smad-signaling pathway
%K Extracellular signal-regulated kinase
%U http://www.biomedcentral.com/1472-6882/12/33/abstract