%0 Journal Article
%T Observation of Esterase-Like-Albumin Activity during N¡¯-Nitrosodimethyl amine Induced Hepatic Fibrosis in a Mammalian Model
%A Areeba Ahmad
%A Veena Maheshwari
%A Arif Ahmad
%A Rashid Saleem
%J Macedonian Journal of Medical Sciences
%@ 1857-5773
%D 2012
%I 
%X Aim: Hepatic fibrosis (HF) is characterized by irregular growth and amassing of fibrous scar tissues in the liver causing weakened hepatocytes metabolism and protein level alterations, including albumin. Albumin with Mr ~68-70 kDa is unglycosylated soluble plasma protein with various biological roles. In this study, we demonstrate ¡®esterase-like activity¡¯ of albumin during NDMA-induced HF in rats.Material and Methods: In rats, HF was induced by weekly i.p. injections of NDMA in doses of 10 mg/kg b.wt. Sera of controls (untreated) and treated rats were processed for biochemical tests, electrophoretic profiling and in-gel esterase activity localization using a, b-naphthyl acetates. H&E staining of liver sections (~ 5 mcm) was done to confirm induction of HF. Results: NDMA satisfactorily induces hepatic fibrosis within 21 days which is also evident by significant increase in SALP, SGOT, SGPT and bilirubin levels in rats. ¡®Esterase-like activity¡¯ of albumin detected in animal sera remains stable throughout the course of treatment irrespective of other biochemical changes. Conclusion: During pathogenesis of HF, formation of stable esterase-albumin complex may have some important role and hence, prior recommending the use of albumin as diagnostic marker we propose further investigations to elucidate the mechanism of its formation.
%K Esterase-like albumin activity
%K Hepatic fibrosis
%K Serum esterases
%K N¡¯-Nitrosodimethyl amine
%K PAGE.
%U http://www.mjms.ukim.edu.mk/Online/MJMS_2012_5_1/MJMS.1857-5773.2012-0214.pdf