%0 Journal Article
%T Enzymatic kinetic method for determination of propranolol hydrochloride in pharmaceuticals based on its inhibitory effect on cholinesterase
%A Stankov-Jovanovi£¿ Vesna P.
%A Miti£¿ Violeta D.
%A Ili£¿ Marija D.
%A Mandi£¿ Ljuba M.
%J Hemijska Industrija
%D 2012
%I Association of Chemical Engineers, Belgrade
%R 10.2298/hemind120128032s
%X Propranolol, a widely used beta-blocker, inhibits the reaction of enzyme cholinesterase hydrolysis. Measurements of the hydrolysis rate differences between and inhibited reactions, allows the development of a kinetic method for its determination. Both systems, enzyme-substrate-chromogen and enzyme-substrate-chromogen-inhibitor, were characterized through biochemical kinetic parameters (KM = 0.326-0.330 mmol/L; Vmax = 40-42.99 ¦̀mol/Lmin), inhibition type was recognized as competitive, and inhibition constant, Ki, was determined to be 22.60 ¦̀mol/L. The detection and quantification limits were calculated as 0.004 and 0.0136 ¦̀mol/L, respectively. Accuracy and precision of proposed methods were tested. Proposed method is characterized with good sensitivity, selectivity, simplicity and rapidity, thus it is convenient for clinical applications.
%K propranolol
%K cholinesterase inhibition
%K kinetic method
%K pharmaceuticals
%U http://www.doiserbia.nb.rs/img/doi/0367-598X/2012/0367-598X1200032S.pdf